Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum

Autor(es)

Santos, Catiule de Oliveira ^[1] ; Costa, Sidnei Ferro ^[2] ; Souza, Fabiana Santana ^[1] ; Ferreira Mendes, Jessica Mariane ^[1] ; Melo de Pinheiro, Cristiane Garboggini ^[1] ; de Magalhaes Moreira, Diogo Rodrigo ^[1] ; Silva, Luciano Kalabric ^[1] ; Felix de Lima, Valeria Marcal ^[2] ; de Sa Oliveira, Geraldo Gileno ^[1]

Afiliação do(s) autor(es)

^[1] Fundacao Oswaldo Cruz, Goncalo Moniz Res Ctr, Lab Struct & Mol Pathol LAPEM, Tissue Engn & Immunopharmacol Lab LETI, Pathol & M, Salvador, BA - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Vet Med, Dept Clin Med Surg & Anim Reprod, Aracatuba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 16, n. 1 JAN 19 2021.
• Citações Web of Science: 0

Resumo

rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum. (AU)

• Processo FAPESP: 17/10906-8 - Regulação da resposta imunológica T1 ou T2 com o uso de citocinas recombinantes em células mononuclerares de cães com Leishmaniose Visceral
• Beneficiário: Sidnei Ferro Costa
• Modalidade de apoio: Bolsas no Brasil - Mestrado