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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum

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Author(s):
Santos, Catiule de Oliveira [1] ; Costa, Sidnei Ferro [2] ; Souza, Fabiana Santana [1] ; Ferreira Mendes, Jessica Mariane [1] ; Melo de Pinheiro, Cristiane Garboggini [1] ; de Magalhaes Moreira, Diogo Rodrigo [1] ; Silva, Luciano Kalabric [1] ; Felix de Lima, Valeria Marcal [2] ; de Sa Oliveira, Geraldo Gileno [1]
Total Authors: 9
Affiliation:
[1] Fundacao Oswaldo Cruz, Goncalo Moniz Res Ctr, Lab Struct & Mol Pathol LAPEM, Tissue Engn & Immunopharmacol Lab LETI, Pathol & M, Salvador, BA - Brazil
[2] Sao Paulo State Univ UNESP, Sch Vet Med, Dept Clin Med Surg & Anim Reprod, Aracatuba - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 16, n. 1 JAN 19 2021.
Web of Science Citations: 0
Abstract

rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum. (AU)

FAPESP's process: 17/10906-8 - Regulation of the T1 or T2 immune response with the use of recombinant cytokines in mononuclear cells of dogs with Visceral Leishmaniasis
Grantee:Sidnei Ferro Costa
Support Opportunities: Scholarships in Brazil - Master