The progression of Canine Visceral Leishmaniasis (CVL) is accompanied by asuppression of cellular immunity probably associated with the inhibition of a cellular response, characterized by a reduction in the number of T lymphocytes and production of the cytokine INF-gamma that suppresses the function of macrophages. Moreover, the PD1 molecule (Programed Cell Death 1) is highly expressed inleukocyte cells in CVL and induces inhibition of the proliferative antigen response and apoptosis of T cells. Cytokines associated with the cell immune response IL-2, IL-12 and INF-³ are highly expressed in different tissues of dogs with asymptomatic leishmaniasis, suggesting that cytokines play a role in disease resistance, while that the IL-10 cytokine associated with humoral response was correlated positively the absence or decrease of INF-³ production and increased parasite load on acute LVC. No studies have yet been performed to evaluate the appropriate conditions for differentiation of the cellular or the ability of the IL-2, IL-12, IL-7 and IL-15 cytokines was not evaluated either to promote lymphoproliferation in healthy and LV dogs. Therefore we will evaluate the combination of canine recombinant reagents IL-2, IL-12, IL-7 and IL-15 and / or soluble receptor of IL-10R (with antagonistic action) is capable to induce differentiation of T1 and T2-like effector T lymphocytes, promote the proliferation of specific antigen T cells and regulate the expression of negative costimulatory molecule PD-1.
News published in Agência FAPESP Newsletter about the scholarship: