Three-dimensional imaging of mitochondrial cristae complexity using cryo-soft X-ray tomography

Autor(es)

Polo, Carla C. ^[1] ; Fonseca-Alaniz, Miriam H. ^[2] ; Chen, Jian-Hua ^{[3, 4]} ; Ekman, Axel ^[3] ; McDermott, Gerry ^[3] ; Meneau, Florian ^[1] ; Krieger, Jose E. ^[2] ; Miyakawa, Ayumi A. ^[2]

Afiliação do(s) autor(es)

^[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Heart Inst InCor, Lab Genet & Mol Cardiol, Sch Med, Sao Paulo, SP - Brazil

^[3] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA - USA

^[4] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94158 - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 DEC 3 2020.
• Citações Web of Science: 0

Resumo

Mitochondria are dynamic organelles that change morphology to adapt to cellular energetic demands under both physiological and stress conditions. Cardiomyopathies and neuronal disorders are associated with structure-related dysfunction in mitochondria, but three-dimensional characterizations of the organelles are still lacking. In this study, we combined high-resolution imaging and 3D electron density information provided by cryo-soft X-ray tomography to characterize mitochondria cristae morphology isolated from murine. Using the linear attenuation coefficient, the mitochondria were identified (0.247 +/- 0.04 mu m(-1)) presenting average dimensions of 0.90 +/- 0.20 mu m in length and 0.63 +/- 0.12 mu m in width. The internal mitochondria structure was successfully identified by reaching up the limit of spatial resolution of 35 nm. The internal mitochondrial membranes invagination (cristae) complexity was calculated by the mitochondrial complexity index (MCI) providing quantitative and morphological information of mitochondria larger than 0.90 mm in length. The segmentation to visualize the cristae invaginations into the mitochondrial matrix was possible in mitochondria with MCI >= 7. Altogether, we demonstrated that the MCI is a valuable quantitative morphological parameter to evaluate cristae modelling and can be applied to compare healthy and disease state associated to mitochondria morphology. (AU)

• Processo FAPESP: 14/25964-5 - Dos monômeros às nanopartículas: investigação in situ da formação de nanopartículas de ouro em tempo real por técnicas síncrotron e de microscopia
• Beneficiário: Florian Edouard Pierre Meneau
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/11139-5 - Participação do CRP3 no processo de aneurisma de aorta abdominal
• Beneficiário: Ayumi Aurea Miyakawa Yamaguchi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/17368-0 - Genômica cardiovascular: mechanismos & novas terapias - CVGen mech2ther
• Beneficiário: José Eduardo Krieger
• Modalidade de apoio: Auxílio à Pesquisa - Temático