| Texto completo | |
| Autor(es): |
Machado, Raquel Moura
[1]
;
de Oliveira, Fernanda Ancelmo
[2]
;
de Matos Alves, Fabio
[2]
;
de Souza, Anete Pereira
[2]
;
Forni-Martins, Eliana Regina
[2]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Inst Biol, Programa Posgrad Biol Vegetal, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOCHEMICAL GENETICS; v. 59, n. 1, p. 219-234, FEB 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Polyploidy is a phenomenon that alters the genetic diversity of populations and has been reported as one of the most important evolutionary forces for plant diversification. ThePsidium cattleyanumcomplex comprises a group of wild populations with several ploidy levels reported in the literature. The multiple cytotypes, associated with its wide distribution area, make this species a potential key model for understanding evolutionary processes related to polyploidization. In this study, we isolated and characterized nuclear microsatellite markers ofP. cattleyanumand tested their transferability to other nine species of the genus. We performed a preliminary analysis of genetic diversity and population structure in three populations ofP. cattleyanum. The three populations analyzed had different chromosome numbers, being polyploid cytotypes (2n = 6x = 66, 2n = 7x = 77 and 2n = 8x = 88). We designed 46 primer pairs and successfully amplified 37 markers, from which the 10 best were selected for analysis. Considering both the PIC and DP values, most of markers were highly informative. The new SSR markers were used to assess the levels of genetic diversity of the populations and detected one population with predominance of sexual reproduction. DAPC analysis pointed the formation of three groups, which corresponded to the populations analyzed. The markers were successfully amplified in related species, with some species presenting 80% transferability. By producing this panel of polymorphic microsatellites, we contribute to the understanding evolution in groups of natural polyploids for future studies. (AU) | |
| Processo FAPESP: | 18/18527-9 - Relações filogenéticas e identificação de marcadores para apomixia e espécie-específicos do grupo Plicatula em genomas poliplóides de Paspalum |
| Beneficiário: | Fernanda Ancelmo de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |