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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Population Genetics of Polyploid ComplexPsidium cattleyanumSabine (Myrtaceae): Preliminary Analyses Based on New Species-Specific Microsatellite Loci and Extension to Other Species of the Genus

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Author(s):
Machado, Raquel Moura [1] ; de Oliveira, Fernanda Ancelmo [2] ; de Matos Alves, Fabio [2] ; de Souza, Anete Pereira [2] ; Forni-Martins, Eliana Regina [2]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Programa Posgrad Biol Vegetal, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOCHEMICAL GENETICS; v. 59, n. 1, p. 219-234, FEB 2021.
Web of Science Citations: 0
Abstract

Polyploidy is a phenomenon that alters the genetic diversity of populations and has been reported as one of the most important evolutionary forces for plant diversification. ThePsidium cattleyanumcomplex comprises a group of wild populations with several ploidy levels reported in the literature. The multiple cytotypes, associated with its wide distribution area, make this species a potential key model for understanding evolutionary processes related to polyploidization. In this study, we isolated and characterized nuclear microsatellite markers ofP. cattleyanumand tested their transferability to other nine species of the genus. We performed a preliminary analysis of genetic diversity and population structure in three populations ofP. cattleyanum. The three populations analyzed had different chromosome numbers, being polyploid cytotypes (2n = 6x = 66, 2n = 7x = 77 and 2n = 8x = 88). We designed 46 primer pairs and successfully amplified 37 markers, from which the 10 best were selected for analysis. Considering both the PIC and DP values, most of markers were highly informative. The new SSR markers were used to assess the levels of genetic diversity of the populations and detected one population with predominance of sexual reproduction. DAPC analysis pointed the formation of three groups, which corresponded to the populations analyzed. The markers were successfully amplified in related species, with some species presenting 80% transferability. By producing this panel of polymorphic microsatellites, we contribute to the understanding evolution in groups of natural polyploids for future studies. (AU)

FAPESP's process: 18/18527-9 - Phylogenetic relationships and identification of markers for apomixis and species-specific Plicatula group in polyploid genomes of Paspalum
Grantee:Fernanda Ancelmo de Oliveira
Support Opportunities: Scholarships in Brazil - Post-Doctoral