| Texto completo | |
| Autor(es): |
Leastro, Mikhail Oliveira
[1, 2]
;
Freitas-Astua, Juliana
[3, 1]
;
Kitajima, Elliot Watanabe
[4]
;
Pallas, Vicente
[2]
;
Sanchez-Navarro, Jesus A.
[2]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Inst Biol, Unidade Lab Referencia Biol Mol Aplicada, Sao Paulo, SP - Brazil
[2] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia - Spain
[3] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA - Brazil
[4] Univ Sao Paulo, Dept Fitopatol & Nematol, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SCIENTIFIC REPORTS; v. 11, n. 1 FEB 3 2021. |
| Citações Web of Science: | 2 |
| Resumo | |
Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. (AU) | |
| Processo FAPESP: | 15/10249-1 - Estudo dos processos infectivos do vírus da leprose do citros (CiLV-C), com ênfase na construção de clone infeccioso e caracterização das proteínas virais. |
| Beneficiário: | Mikhail Oliveira Leastro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/50222-0 - Compreensão sobre a biologia molecular e ecologia da relações planta-vírus-vetor: rumo a estratégias sustentáveis e integradas de manejo de vírus |
| Beneficiário: | Juliana de Freitas Astúa |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/19898-8 - O envolvimento da proteína de movimento (MP) dos vírus associados com a leprose do citros com a limitação natural do movimento viral sistêmico na planta. |
| Beneficiário: | Mikhail Oliveira Leastro |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |