Unravelling the involvement of cilevirus p32 protein in the viral transport

Autor(es)

Leastro, Mikhail Oliveira ^{[1, 2]} ; Freitas-Astua, Juliana ^{[3, 1]} ; Kitajima, Elliot Watanabe ^[4] ; Pallas, Vicente ^[2] ; Sanchez-Navarro, Jesus A. ^[2]

Afiliação do(s) autor(es)

^[1] Inst Biol, Unidade Lab Referencia Biol Mol Aplicada, Sao Paulo, SP - Brazil

^[2] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia - Spain

^[3] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA - Brazil

^[4] Univ Sao Paulo, Dept Fitopatol & Nematol, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 FEB 3 2021.
• Citações Web of Science: 1

Resumo

Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. (AU)

• Processo FAPESP: 17/50222-0 - Compreensão sobre a biologia molecular e ecologia da relações planta-vírus-vetor: rumo a estratégias sustentáveis e integradas de manejo de vírus
• Beneficiário: Juliana de Freitas Astúa
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/19898-8 - O envolvimento da proteína de movimento (MP) dos vírus associados com a leprose do citros com a limitação natural do movimento viral sistêmico na planta.
• Beneficiário: Mikhail Oliveira Leastro
• Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 15/10249-1 - Estudo dos processos infectivos do vírus da leprose do citros (CiLV-C), com ênfase na construção de clone infeccioso e caracterização das proteínas virais.
• Beneficiário: Mikhail Oliveira Leastro
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado