Omega-3 Polyunsaturated Fatty Acids Prevent Nonalcoholic Steatohepatitis (NASH) and Stimulate Adipogenesis

Autor(es)

Antraco, Vitor Jaco ^[1] ; Hirata, Bruna Kelly Sousa ^[1] ; de Jesus Simao, Jussara ^[1] ; Cruz, Maysa Mariana ^[1] ; da Silva, Viviane Simoes ^[1] ; da Cunha de Sa, Roberta Dourado Cavalcante ^[1] ; Abdala, Fernanda Miranda ^[1] ; Armelin-Correa, Lucia ^[1] ; Alonso-Vale, Maria Isabel Cardoso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Biol Sci, Inst Environm Sci Chem & Pharmaceut, BR-09913130 Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRIENTS; v. 13, n. 2 FEB 2021.
• Citações Web of Science: 5

Resumo

The increasing impact of obesity on global human health intensifies the importance of studies focusing on agents interfering with the metabolism and remodeling not only of the white adipose tissue (WAT) but also of the liver. In the present study, we have addressed the impact of n-3 PUFA in adipose cells' proliferation and adipogenesis, as well as in the hepatic lipid profile and morphology. Mice were induced to obesity by the consumption of a high-fat diet (HFD) for 16 weeks. At the 9th week, the treatment with fish oil (FO) was initiated and maintained until the end of the period. The FO treatment reduced the animals' body mass, plasma lipids, glucose, plasma transaminases, liver mass, triacylglycerol, and cholesterol liver content when compared to animals consuming only HFD. FO also decreased the inguinal (ing) WAT mass, reduced adipocyte volume, increased adipose cellularity (hyperplasia), and increased the proliferation of adipose-derived stromal cells (AdSCs) which corroborates the increment in the proliferation of 3T3-L1 pre-adipocytes or AdSCs treated in vitro with n-3 PUFA. After submitting the in vitro treated (n-3 PUFA) cells, 3T3-L1 and AdSCs, to an adipogenic cocktail, there was an increase in the mRNA expression of adipogenic transcriptional factors and other late adipocyte markers, as well as an increase in lipid accumulation when compared to not treated cells. Finally, the expression of browning-related genes was also higher in the n-3 PUFA treated group. We conclude that n-3 PUFA exerts an attenuating effect on body mass, dyslipidemia, and hepatic steatosis induced by HFD. FO treatment led to decreasing adiposity and adipocyte hypertrophy in ingWAT while increasing hyperplasia. Data suggest that FO treatment might induce recruitment (by increased proliferation and differentiation) of new adipocytes (white and/or beige) to the ingWAT, which is fundamental for the healthy expansion of WAT. (AU)

• Processo FAPESP: 18/05485-6 - Efeitos da dieta hiperlipídica e da suplementação com ácidos graxos ômega-7 e ômega-3 sobre o processo de browning, proliferação e diferenciação de células mesenquimais contidas na fração celular estromal do tecido adiposo
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Regular