Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia scierotiorum

Autor(es)

Guilger-Casagrande, Mariana ^{[1, 2]} ; Germano-Costa, Tais ^[1] ; Bilesky-Jose, Natalia ^[1] ; Pasquoto-Stigliani, Tatiane ^[1] ; Carvalho, Lucas ^[2] ; Fraceto, Leonardo F. ^[2] ; de Lima, Renata ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sorocaba, Lab Evaluat Bioact & Toxicol Nanomat, Sorocaba, SP - Brazil

^[2] Sao Paulo State Univ, Lab Environm Nanotechnol, Sorocaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NANOBIOTECHNOLOGY; v. 19, n. 1 FEB 24 2021.
• Citações Web of Science: 0

Resumo

Background: Biogenic nanoparticles possess a capping of biomolecules derived from the organism employed in the synthesis, which contributes to their stability and biological activity. These nanoparticles have been highlighted for the control of phytopathogens, so there is a need to understand their composition, mechanisms of action, and toxicity. This study aimed to investigate the importance of the capping and compare the effects of capped and uncapped biogenic silver nanoparticles synthesized using the filtrate of Trichoderma harzianum against the phytopathogenic fungus Sclerotinia sclerotiorum. Capping removal, investigation of the composition of the capping and physico-chernical characterization of the capped and uncapped nanoparticles were performed. The effects of the nanoparticles on S. scierotiorum were evaluated in vitro. Cytotoxicity and genotoxicity of the nanoparticles on different cell lines and its effects on nontarget microorganisms were also investigated. Results: The capped and uncapped nanoparticles showed spherical morphology, with greater diameter of the uncapped ones. Functional groups of biomolecules, protein bands and the hydrolytic enzymes NAGase, beta-1,3-glucanase, chitinase and acid protease from T. harzianum were detected in the capping. The capped nanoparticles showed great inhibitory potential against S. scierotiorum, while the uncapped nanoparticles were ineffective. There was no difference in cytotoxicity comparing capped and uncapped nanoparticles, however higher genotoxicity of the uncapped nanoparticles was observed towards the cell lines. Regarding the effects on nontarget microorganisms, in the minimal inhibitory concentration assay only the capped nanoparticles inhibited microorganisms of agricultural importance, while in the molecular analysis of the soil microbiota there were major changes in the soils exposed to the uncapped nanoparticles. Conclusions: The results suggest that the capping played an important role in controlling nanoparticle size and contributed to the biological activity of the nanoparticles against S. scierotiorum. This study opens perspectives for investigations concerning the application of these nanoparticles for the control of phytopathogens. (AU)

• Processo FAPESP: 16/13692-6 - Nanopartículas biogênicas de prata baseadas em Trichoderma harzianum: diferentes condições de síntese, avaliação da cito/genotoxicidade e potencial inibitório de Sclerotinia sclerotiorum
• Beneficiário: Mariana Guilger Casagrande
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/23608-8 - Desenvolvimento de nanopesticidas marcados com sondas fluorescentes para estudos de mecanismo de ação e toxicidade
• Beneficiário: Lucas Bragança de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/20932-6 - Síntese verde e avaliação de nanopartículas de ferro baseadas em Trichoderma harzianum visando aplicação na agricultura
• Beneficiário: Natalia Bilesky José
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/13328-5 - Desenvolvimento e avaliação de nanopartiulas metálicas biogênicas (prata, titânio e ferro) baseadas em Trichoderma harzianum para aplicação agrícola
• Beneficiário: Renata de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/05816-2 - Estudo do recobrimento (capping) de nanopartículas biogênicas e sua correlação com toxicidade e atividade no controle de fitopatógenos
• Beneficiário: Mariana Guilger Casagrande
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/21004-5 - Agricultura, micro/nanotecnologia e ambiente: da avaliação dos mecanismos de ação a estudos de transporte e toxicidade
• Beneficiário: Leonardo Fernandes Fraceto
• Modalidade de apoio: Auxílio à Pesquisa - Temático