| Texto completo | |
| Autor(es): |
Dip Gandarilla, Ariamna Maria
[1]
;
Regiart, Matias
[2]
;
Bertotti, Mauro
[2]
;
Correa Gloria, Juliane
[3]
;
Morais Mariuba, Luis Andre
[3]
;
Brito, Walter Ricardo
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Fed Amazonas, Dept Chem, BR-69067005 Manaus, Amazonas - Brazil
[2] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, BR-05508000 Sao Paulo - Brazil
[3] Fundacao Oswaldo Cruz, Leonidas & Maria Deane Inst, BR-69057070 Manaus, Amazonas - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | RSC ADVANCES; v. 11, n. 1, p. 408-415, JAN 1 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
In the present work, we describe a novel one-step enzyme-free dual electrochemical immunosensor for the determination of histidine-rich protein 2 (Ag-PfHRP2), a specific malaria biomarker. A gold electrode (GE) was functionalized with the PfHRP2 antibody (Ab-PfHRP2) using dihexadecyl phosphate (DHP) polymer as an immobilization platform. The Ab-PfHRP2/DHP/GE sensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The developed immunosensor was employed for indirect Ag-PfHRP2 determination by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The linear range was 10-400 ng mL(-1) and 10-500 ng mL(-1) for EIS and DPV, while the limit of detection was 3.3 ng mL(-1) and 2.8 ng mL(-1), respectively. The electrochemical immunosensor was successfully applied for Ag-PfHRP2 determination in human serum samples. Its performance was compared with an ELISA test, and good correspondence was achieved. The coefficients of intra- and inter-assay variations were less than 5%. The electrochemical immunosensor is a useful and straightforward tool for in situ malaria biomarker determination. (AU) | |
| Processo FAPESP: | 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais |
| Beneficiário: | Mauro Bertotti |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/06293-6 - Investigações em sistemas biológicos empregando sensores eletroquímicos miniaturizados |
| Beneficiário: | Daniel Matias Gaston Regiart |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |