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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination

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Author(s):
Dip Gandarilla, Ariamna Maria [1] ; Regiart, Matias [2] ; Bertotti, Mauro [2] ; Correa Gloria, Juliane [3] ; Morais Mariuba, Luis Andre [3] ; Brito, Walter Ricardo [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Amazonas, Dept Chem, BR-69067005 Manaus, Amazonas - Brazil
[2] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, BR-05508000 Sao Paulo - Brazil
[3] Fundacao Oswaldo Cruz, Leonidas & Maria Deane Inst, BR-69057070 Manaus, Amazonas - Brazil
Total Affiliations: 3
Document type: Journal article
Source: RSC ADVANCES; v. 11, n. 1, p. 408-415, JAN 1 2021.
Web of Science Citations: 0
Abstract

In the present work, we describe a novel one-step enzyme-free dual electrochemical immunosensor for the determination of histidine-rich protein 2 (Ag-PfHRP2), a specific malaria biomarker. A gold electrode (GE) was functionalized with the PfHRP2 antibody (Ab-PfHRP2) using dihexadecyl phosphate (DHP) polymer as an immobilization platform. The Ab-PfHRP2/DHP/GE sensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The developed immunosensor was employed for indirect Ag-PfHRP2 determination by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The linear range was 10-400 ng mL(-1) and 10-500 ng mL(-1) for EIS and DPV, while the limit of detection was 3.3 ng mL(-1) and 2.8 ng mL(-1), respectively. The electrochemical immunosensor was successfully applied for Ag-PfHRP2 determination in human serum samples. Its performance was compared with an ELISA test, and good correspondence was achieved. The coefficients of intra- and inter-assay variations were less than 5%. The electrochemical immunosensor is a useful and straightforward tool for in situ malaria biomarker determination. (AU)

FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
Grantee:Mauro Bertotti
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/06293-6 - Investigations in biological systems by using miniaturized electrochemical sensors
Grantee:Daniel Matias Gaston Regiart
Support Opportunities: Scholarships in Brazil - Post-Doctoral