Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro

Autor(es)

Soares, Matheus Fujimura ^[1] ; Melo, Larissa Martins ^[1] ; Bragato, Jaqueline Poleto ^[1] ; Furlan, Amanda de Oliveira ^[2] ; Scaramele, Natalia Francisco ^[2] ; Lopes, Flavia Lombardi ^[2] ; Felix de Lima, Valeria Marcal ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Vet Med, Dept Surg & Anim Reprod, 793 Clovis Pestana St, BR-16050680 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Vet Med, Dept Support Prod & Anim Hlth, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Research in Veterinary Science; v. 134, p. 58-63, JAN 2021.
• Citações Web of Science: 0

Resumo

Visceral Leishmaniasis (VL) is a neglected tropical disease, caused by L. infantum in the New World, where dogs are the main reservoir. These parasites can regulate host immune response through miRNA differential expression in the early stages of infection; however such early response has not yet been investigated in the canine model. PBMC from healthy dogs were exposed to L. infantum in vitro and microarray analysis showed an upregulation of miR-206, miR-302d, miR-433, miR-214, miR-493, miR-514, miR-1835, miR-210, miR-539, miR432, miR-188, miR-345 and downregulation of miR-489 and miR-503 in comparison to non-exposed control cells, at 24 h post-exposure. In silico target prediction showed that the upregulated miRNAs target 1541 genes, which can modulate important pathways involved in the early immune responses, like the ``MAPK signaling pathway{''}, one of the most relevant pathways to Leishmania survival inside host cells. These findings shed light on parasite modulation of host immunity following Leishmania infection, which in turn can be explored for drug development. (AU)

• Processo FAPESP: 15/16101-6 - O papel dos miRNAs na infecção de células mononucleares de sangue periférico e de leucócitos esplênicos de cão por Leishmania infantum
• Beneficiário: Larissa Martins Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/16972-7 - O papel dos miRNA na infecção de células mononucleares de sangue e baço de cão por Leishmania infantum
• Beneficiário: Valéria Marçal Felix de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular