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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro

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Author(s):
Soares, Matheus Fujimura [1] ; Melo, Larissa Martins [1] ; Bragato, Jaqueline Poleto [1] ; Furlan, Amanda de Oliveira [2] ; Scaramele, Natalia Francisco [2] ; Lopes, Flavia Lombardi [2] ; Felix de Lima, Valeria Marcal [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Vet Med, Dept Surg & Anim Reprod, 793 Clovis Pestana St, BR-16050680 Aracatuba, SP - Brazil
[2] Sao Paulo State Univ Unesp, Sch Vet Med, Dept Support Prod & Anim Hlth, Aracatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Research in Veterinary Science; v. 134, p. 58-63, JAN 2021.
Web of Science Citations: 0
Abstract

Visceral Leishmaniasis (VL) is a neglected tropical disease, caused by L. infantum in the New World, where dogs are the main reservoir. These parasites can regulate host immune response through miRNA differential expression in the early stages of infection; however such early response has not yet been investigated in the canine model. PBMC from healthy dogs were exposed to L. infantum in vitro and microarray analysis showed an upregulation of miR-206, miR-302d, miR-433, miR-214, miR-493, miR-514, miR-1835, miR-210, miR-539, miR432, miR-188, miR-345 and downregulation of miR-489 and miR-503 in comparison to non-exposed control cells, at 24 h post-exposure. In silico target prediction showed that the upregulated miRNAs target 1541 genes, which can modulate important pathways involved in the early immune responses, like the ``MAPK signaling pathway{''}, one of the most relevant pathways to Leishmania survival inside host cells. These findings shed light on parasite modulation of host immunity following Leishmania infection, which in turn can be explored for drug development. (AU)

FAPESP's process: 15/16101-6 - The role of miRNAs in mononuclear cells infection of peripheral blood and spleen leukocytes the dog by Leishmania infantum
Grantee:Larissa Martins Melo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/16972-7 - The role of miRNA in Leishmania infantum infection in mononuclear cells and spleen leukocytes from peripheral blood of dog
Grantee:Valéria Marçal Felix de Lima
Support Opportunities: Regular Research Grants