Cyclotides from Brazilian Palicourea sessilis and Their Effects on Human Lymphocytes

Autor(es)

Pinto, Meri Emili F. ^{[1, 2]} ; Chan, Lai Yue ^[2] ; Koehbach, Johannes ^[2] ; Devi, Seema ^[3] ; Grundemann, Carsten ^[4] ; Gruber, Christian W. ^[5] ; Gomes, Mario ^[6] ; Bolzani, Vanderlan S. ^[1] ; Cilli, Eduardo Maffud ^[1] ; Craik, David J. ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Chem, BR-14800060 Araraquara, SP - Brazil

^[2] Univ Queensland, Inst Mol Biosci, Australian Res Council Ctr Excellence Innovat Pep, Brisbane, Qld 4072 - Australia

^[3] Univ Freiburg, Inst Infect Prevent & Hosp Epidemiol, Ctr Complementary Med, D-79111 Freiburg - Germany

^[4] Univ Basel, Dept Pharmaceut Sci, Translat Complementary Med, CH-4056 Basel - Switzerland

^[5] Med Univ Vienna, Ctr Physiol & Pharmacol, A-1090 Vienna - Austria

^[6] Rio de Janeiro Bot Garden Res Inst JBRJ, BR-22470180 Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Natural Products; v. 84, n. 1, p. 81-90, JAN 22 2021.
• Citações Web of Science: 2

Resumo

Cyclotides are plant-derived peptides found within five families of flowering plants (Violaceae, Rubiaceae, Fabaceae, Solanaceae, and Poaceae) that have a cyclic backbone and six conserved cysteine residues linked by disulfide bonds. Their presence within the Violaceae species seems ubiquitous, yet not all members of other families produce these macrocyclic peptides. The genus Palicourea Aubl. (Rubiaceae) contains hundreds of neotropical species of shrubs and small trees; however, only a few cyclotides have been discovered hitherto. Herein, five previously uncharacterized Mobius cyclotides within Palicourea sessilis and their pharmacological activities are described. Cyclotides were isolated from leaves and stems of this plant and identified as pase A-E, as well as the known peptide kalata S. Cyclotides were de novo sequenced by MALDI-TOF/TOF mass spectrometry, and their structures were solved by NMR spectroscopy. Because some cyclotides have been reported to modulate immune cells, pase A-D were assayed for cell proliferation of human primary activated T lymphocytes, and the results showed a dose-dependent antiproliferative function. The toxicity on other nonimmune cells was also assessed. This study reveals that pase cyclotides have potential for applications as immunosuppressants and in immune-related disorders. (AU)

• Processo FAPESP: 19/04381-5 - Últimos Avanços em Síntese em Fase-sólida e Novas metodologias de RMN aplicadas aos Ciclotídeos de Plantas Brasileiras
• Beneficiário: Meri Emili Pinto Nascimento
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 17/17098-4 - Emprego de imageamento MALDI-MSI em tecidos foliares nodulados e não nodulados de Psychotria e Hybanthus: Localização, análise de ciclotídeos e sua relação com bactérias endossimbiontes
• Beneficiário: Meri Emili Pinto Nascimento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
• Beneficiário: Glaucius Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs