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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cyclotides from Brazilian Palicourea sessilis and Their Effects on Human Lymphocytes

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Author(s):
Pinto, Meri Emili F. [1, 2] ; Chan, Lai Yue [2] ; Koehbach, Johannes [2] ; Devi, Seema [3] ; Grundemann, Carsten [4] ; Gruber, Christian W. [5] ; Gomes, Mario [6] ; Bolzani, Vanderlan S. [1] ; Cilli, Eduardo Maffud [1] ; Craik, David J. [2]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Chem, BR-14800060 Araraquara, SP - Brazil
[2] Univ Queensland, Inst Mol Biosci, Australian Res Council Ctr Excellence Innovat Pep, Brisbane, Qld 4072 - Australia
[3] Univ Freiburg, Inst Infect Prevent & Hosp Epidemiol, Ctr Complementary Med, D-79111 Freiburg - Germany
[4] Univ Basel, Dept Pharmaceut Sci, Translat Complementary Med, CH-4056 Basel - Switzerland
[5] Med Univ Vienna, Ctr Physiol & Pharmacol, A-1090 Vienna - Austria
[6] Rio de Janeiro Bot Garden Res Inst JBRJ, BR-22470180 Rio De Janeiro, RJ - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Journal of Natural Products; v. 84, n. 1, p. 81-90, JAN 22 2021.
Web of Science Citations: 2
Abstract

Cyclotides are plant-derived peptides found within five families of flowering plants (Violaceae, Rubiaceae, Fabaceae, Solanaceae, and Poaceae) that have a cyclic backbone and six conserved cysteine residues linked by disulfide bonds. Their presence within the Violaceae species seems ubiquitous, yet not all members of other families produce these macrocyclic peptides. The genus Palicourea Aubl. (Rubiaceae) contains hundreds of neotropical species of shrubs and small trees; however, only a few cyclotides have been discovered hitherto. Herein, five previously uncharacterized Mobius cyclotides within Palicourea sessilis and their pharmacological activities are described. Cyclotides were isolated from leaves and stems of this plant and identified as pase A-E, as well as the known peptide kalata S. Cyclotides were de novo sequenced by MALDI-TOF/TOF mass spectrometry, and their structures were solved by NMR spectroscopy. Because some cyclotides have been reported to modulate immune cells, pase A-D were assayed for cell proliferation of human primary activated T lymphocytes, and the results showed a dose-dependent antiproliferative function. The toxicity on other nonimmune cells was also assessed. This study reveals that pase cyclotides have potential for applications as immunosuppressants and in immune-related disorders. (AU)

FAPESP's process: 19/04381-5 - The Last Advances on Solid-Phase Peptide Synthesis and NMR New Methodologies Applied to Cyclotides from Brazilian Plant Species
Grantee:Meri Emili Pinto Nascimento
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 17/17098-4 - Application of MALDI Imaging (MSI) on non-nodulated and nodulated leaves of Psychotria and Hybanthus: Localization, analysis of cyclotides and its relationship with endosymbionts bacteria
Grantee:Meri Emili Pinto Nascimento
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
Grantee:Glaucius Oliva
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC