Dysmorphic neurons as cellular source for phase-amplitude coupling in Focal Cortical Dysplasia Type II

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Rampp, Stefan ^{[1, 2]} ; Rossler, Karl ^{[1, 3]} ; Hamer, Hajo ^[4] ; Illek, Margit ^[1] ; Buchfelder, Michael ^[1] ; Doerfler, Arnd ^[5] ; Pieper, Tom ^[6] ; Hartlieb, Till ^[6] ; Kudernatsch, Manfred ^{[7, 8, 9]} ; Koelble, Konrad ^[10] ; Peixoto-Santos, Jose Eduardo ^{[10, 11]} ; Blumcke, Ingmar ^[10] ; Coras, Roland ^[10]

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^[1] Univ Hosp Erlangen, Dept Neurosurg, Schwabachanlage 6, D-91054 Erlangen - Germany

^[2] Univ Hosp Halle, Dept Neurosurg, Halle - Germany

^[3] Univ Hosp Vienna, Dept Neurosurg, Vienna - Austria

^[4] Univ Hosp Erlangen, Epilepsy Ctr, Dept Neurol, Erlangen - Germany

^[5] Univ Hosp Erlangen, Dept Neuroradiol, Erlangen - Germany

^[6] Hosp Neuropediat & Neurol Rehabil, Epilepsy Ctr Children & Adolescents, Schon Klin Vogtareuth, Vogtareuth - Germany

^[7] Schon Klin Vogtareuth, Epilepsy Ctr, Vogtareuth - Germany

^[8] Schon Klin Vogtareuth, Dept Neurosurg, Vogtareuth - Germany

^[9] PMU Salzburg, Res Inst Rehabil Transit Palliat, Salzburg - Austria

^[10] Univ Hosp Erlangen, Dept Neuropathol, Erlangen - Germany

^[11] Univ Fed Sao Paulo, Dept Neurol & Neurosurg, Paulista Sch Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL NEUROPHYSIOLOGY; v. 132, n. 3, p. 782-792, MAR 2021.
• Citações Web of Science: 0

Resumo

Objective: Reliable localization of the epileptogenic zone is necessary for successful epilepsy surgery. Neurophysiological biomarkers include ictal onsets and interictal spikes. Furthermore, the epileptic network shows oscillations with potential localization value and pathomechanistic implications. The cellular origin of such markers in invasive EEG in vivo remains to be clarified. Methods: In the presented pilot study, surgical brain samples and invasive EEG recordings of seven patients with surgically treated Focal Cortical Dysplasia (FCD) type II were coregistered using a novel protocol. Dysmorphic neurons and balloon cells were immunohistochemically quantified. Evaluated markers included seizure onset, spikes, and oscillatory activity in delta, theta, gamma and ripple frequency bands, as well as sample entropy and phase-amplitude coupling between delta, theta, alpha and beta phase and gamma amplitude. Results: Correlations between histopathology and neurophysiology provided evidence for a contribution of dysmorphic neurons to interictal spikes, fast gamma activity and ripples. Furthermore, seizure onset and phase-amplitude coupling in areas with dysmorphic neurons suggests preserved connectivity is related to seizure initiation. Balloon cells showed no association. Conclusions: Phase-amplitude coupling, spikes, fast gamma and ripples are related to the density of dysmorphic neurons and localize the seizure onset zone. Significance: The results of our pilot study provide a new powerful tool to address the cellular source of abnormal neurophysiology signals to leverage current and novel biomarkers for the localization of epileptic activity in the human brain. (C) 2021 International Federation of Clinical Neurophysiology. Published by Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/03739-8 - Correlatos histológicos de imagens por Ressonância Magnética em pacientes com epilepsias
• Beneficiário: José Eduardo Peixoto Santos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado