Transcranial ultrasonography as a reliable instrument for the measurement of the cerebral ventricles in rats with experimental hydrocephalus: a pilot study

Autor(es)

Pinto de Moura Silva, Gabriel Aparecido ^[1] ; da Silva, Stephanya Covas ^[2] ; Beggiora, Pamella da Silva ^[2] ; Matias Junior, Ivair ^{[3, 4]} ; Menezes-Reis, Rafael ^[5] ; Santos, Marcelo Volpon ^[2] ; Machado, Helio Rubens ^[2] ; Lopes, Luiza da Silva ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Coll Nursing, Bandeirantes Av 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Bandeirantes Av 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Lab Neuroanat & Neuropsychobiol, Bandeirantes Av 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Claretiano Ctr Univ, Dom Bosco St 466, BR-14300000 Batatais, SP - Brazil

^[5] Univ Fed Amazonas, Inst Hlth & Biotechnol, Coari Mamia Rd 305, BR-69460000 Coari, AM - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CHILD'S NERVOUS SYSTEM; v. 37, n. 6 FEB 2021.
• Citações Web of Science: 0

Resumo

Purposes Demonstrate that transcranial ultrasonography (TUS) scanning is viable and useful as a diagnostic method in experimental hydrocephalus, as well as to compare measurements of cerebral and ventricular width obtained from TUS scans of hydrocephalic rats with post-mortem anatomical specimens, aiming for the development of accurate criteria to establish ventricular enlargement and progression of hydrocephalus subsequently. Methods Thirty-five male Wistar rats were used. Following hydrocephalus induction, they underwent a transcranial ultrasound scan to measure cerebral and ventricular dimensions, in the fourth and 21 post-induction days. By the end of the experiments, measurements obtained from TUS scans were compared with actual values as seen in the post-mortem specimens of each animal. Results Ventricular dilation could be clearly visualized in hydrocephalic animals. We performed intraclass correlation coefficient and linear regression analyses that have demonstrated a precise correlation between measurements of TUS scans and post-mortem specimens; we have found a similarity of 0,95 for the cerebral diameter and 0,97 for ventricular width. Conclusions Transcranial ultrasonography is a useful and reliable diagnostic tool for experimental hydrocephalus; also, it can be used to assess the progression of ventriculomegaly in animal models of hydrocephalus. (AU)

• Processo FAPESP: 09/54010-1 - EMU: sepsis e choque séptico: alterações funcionais e morfológicas do coração. Estudo experimental em camundongos
• Beneficiário: Helio Cesar Salgado
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 16/11212-7 - Papel da oxigenoterapia hiperbárica no controle de lesões cerebrais da hidrocefalia experimental
• Beneficiário: Luiza da Silva Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Regular