| Texto completo | |
| Autor(es): Mostrar menos - |
Britto-Junior, Jose
[1]
;
Jacintho, Felipe Fernandes
[1]
;
Campos, Rafael
[2]
;
Araujo Pinheiro, David Halen
[1]
;
Figueiredo Murari, Guilherme M.
[1]
;
de Souza, Valeria B.
[1]
;
Schenka, Andre A.
[1]
;
Monica, Fabiola Z.
[1]
;
Moreno, Ronilson Agnaldo
[1]
;
Antunes, Edson
[1]
;
De Nucci, Gilberto
[1, 3]
Número total de Autores: 11
|
| Afiliação do(s) autor(es): | [1] Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, BR-13083894 Campinas - Brazil
[2] Ceara State Univ UECE, Super Inst Biomed Sci, Dept Physiol, BR-60714903 Fortaleza, Ceara - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508060 Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOLOGY OPEN; v. 10, n. 1 JAN 2021. |
| Citações Web of Science: | 1 |
| Resumo | |
The contractions of Chelonoidis carbonaria aortic rings induced by electrical field stimulation (EFS) are not inhibited by blockade of the voltage-gated sodium channels by tetrodotoxin but almost abolished by the alpha 1/alpha 2-adrenoceptor antagonist phentolamine. The objective of this study was to identify the mediator(s) responsible for the EFS-induced contractions of Chelonoidis carbonaria aortic rings. Each ring was suspended between two wire hooks and mounted in isolated 10 ml organ chambers filled with oxygenated and heated Krebs-Henseleit's solution. Dopamine, noradrenaline and adrenaline concentrations were analysed by liquid chromatography coupled to tandem mass spectrometry. The contractions caused by dopamine and EFS were done in absence and presence of the nitric oxide (NO) synthesis inhibitor L-NAME, the NO-sensitive guanylyl cyclase inhibitor ODQ, the D1-like receptor antagonist SCH-23390, the D2-like receptor antagonists risperidone, quetiapine, haloperidol, and the tyrosine hydroxylase inhibitors salsolinol and 3-iodo-L-tyrosine. Basal concentrations of dopamine, noradrenaline and adrenaline were detected in Krebs-Henseleit solution containing the aortic rings. The catecholamine concentrations were significantly reduced in endothelium-denuded aortic rings. L-NAME and ODQ significantly potentiated the dopamine-induced contractions. The D2-like receptor antagonists inhibited the EFS-induced contractions of the aortic rings treated with L-NAME, whereas SCH 23390 had no effect. Similar results were observed in the contractions induced by dopamine in L-NAME treated aortic rings. These results indicate that catecholamines released by endothelium regulate the EFS-induced contractions. This may constitute a suitable mechanism by which reptilia modulate specific organ blood flow distribution. This paper has an associated First Person interview with the first author of the article. (AU) | |
| Processo FAPESP: | 18/24971-9 - Relevância fisiológica das catecolaminas produzidas pelo endotélio |
| Beneficiário: | Felipe Fernandes Jacintho |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 16/04731-8 - Atividade antineoplásica e anti-célula tronco tumoral in vitro e in vivo de sinvastatina, óleo de Copaifera reticulata Ducke e nanopartículas inéditas de óxido de grafeno e de magnetita, em uso isolado ou combinado, em linhagens de carcinoma mamário |
| Beneficiário: | André Almeida Schenka |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/15175-1 - Modulação da guanilato ciclase solúvel e dos níveis intracelulares de nucleotídeos cíclicos em órgãos do trato urinário inferior e próstata |
| Beneficiário: | Edson Antunes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |