Oestrogen replacement fails to fully revert ovariectomy-induced changes in adipose tissue monoglycerides, diglycerides and cholesteryl esters of rats fed a lard-enriched diet

Autor(es)

Boldarine, Valter Tadeu ^[1] ; Joyce, Ellen ^[2] ; Pedroso, Amanda Paula ^[1] ; Telles, Monica Marques ^[1] ; Oyama, Lila Missae ^[1] ; Bueno, Allain Amador ^[2] ; Ribeiro, Eliane Beraldi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Fisiol, Rua Botucatu 862, 2 Andar, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Worcester, Dept Biol Sci, Coll Hlth Life & Environm Sci, Worcester - England

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 FEB 15 2021.
• Citações Web of Science: 0

Resumo

Menopause may be accompanied by abdominal obesity and inflammation, conditions accentuated by high-fat intake, especially of saturated fat (SFA)-rich diets. We investigated the consequences of high-SFA intake on the fatty acid (FA) profile of monoglycerides, diglycerides and cholesteryl esters from retroperitoneal white adipose tissue (RET) of rats with ovariectomy-induced menopause, and the effect of oestradiol replacement. Wistar rats were either ovariectomized (Ovx) or sham operated (Sham) and fed either standard chow (C) or lard-enriched diet (L) for 12 weeks. Half of the Ovx rats received 17 beta -oestradiol replacement (Ovx+E2). Body weight and food intake were measured weekly. RET neutral lipids were chromatographically separated and FAs analysed by gas chromatography. Ovariectomy alone increased body weight, feed efficiency, RET mass, leptin and insulin levels, leptin/adiponectin ratio, HOMA-IR and HOMA-beta indexes. OvxC+E2 showed attenuation in nearly all blood markers. HOMA-beta index was restored in OvxL+E2. OvxC showed significantly disturbed SFA and polyunsaturated FA (PUFA) profile in RET cholesteryl esters (CE). OvxC also showed increased monounsaturated FA (MUFA) in the monoglyceride diglyceride (Mono-Di) fraction. Similar changes were not observed in OvxL, although increased SFA and decreased PUFA was observed in Mono-Di. Overall, HRT was only partially able to revert changes induced by ovariectomy. There appears to be increased mobilization of essential FA in Ovx via CE, which is a dynamic lipid species. The same results were not found in Mono-Di, which are more inert. HRT may be helpful to preserve FA profile in visceral fat, but possibly not wholly sufficient in reverting the metabolic effects induced by menopause. (AU)

• Processo FAPESP: 12/03172-4 - Obesidade e depressão na pós-menopausa: estudo em ratas dos efeitos da ovariectomia sobre aspectos comportamentais e da regulação central da ingestão alimentar
• Beneficiário: Eliane Beraldi Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular