Silane-Coating Strategy for Titanium Functionalization Does Not Impair Osteogenesis In Vivo

Autor(es)

Senna, Plinio Mendes ^[1] ; de Almeida Barros Mourao, Carlos Fernando ^[2] ; Mello-Machado, Rafael Coutinho ^[3] ; Javid, Kayvon ^[3] ; Montemezzi, Pietro ; Del Bel Cury, Altair Antoninha ^[4] ; Meirelles, Luiz ^[5]

Afiliação do(s) autor(es)

^[1] Unigranrio Univ, Hlth Sci Ctr, BR-25071202 Rio De Janeiro - Brazil

^[2] Univ Fed Fluminense, Biotechnol Dept, BR-24070035 Niteroi, RJ - Brazil

^[3] Univ Fed Fluminense, Sch Dent, Grad Program, BR-24020140 Niteroi, RJ - Brazil

^[4] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, BR-13414903 Piracicaba - Brazil

^[5] Ohio State Univ, Dept Restorat & Prosthet Dent, Coll Dent, Columbus, OH 43210 - USA

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS; v. 14, n. 7 APR 2021.
• Citações Web of Science: 0

Resumo

Silane-coating strategy has been used to bind biological compounds to the titanium surface, thereby making implant devices biologically active. However, it has not been determined if the presence of the silane coating itself is biocompatible to osseointegration. The aim of the present study was to evaluate if silane-coating affects bone formation on titanium using a rabbit model. For this, titanium screw implants (3.75 by 6 mm) were hydroxylated in a solution of H2SO4/30% H2O2 for 4 h before silane-coating with 3-aminopropyltriethoxysilane (APTES). A parallel set of titanium screws underwent only the hydroxylation process to present similar acid-etched topography as a control. The presence of the silane on the surface was checked by x-ray photoelectron spectroscopy (XPS), with scanning electron microscopy (SEM) and atomic force microscopy (AFM). A total of 40 titanium screws were implanted in the tibia of ten New Zealand rabbits in order to evaluate bone-to-implant contact (BIC) after 3 weeks and 6 weeks of healing. Silane-coated surface presented higher nitrogen content in the XPS analysis, while micro- and nano-topography of the surface remained unaffected. No difference between the groups was observed after 3 and 6 weeks of healing (p > 0.05, independent t-test), although an increase in BIC occurred over time. These results indicate that silanization of a titanium surface with APTES did not impair the bone formation, indicating that this can be a reliable tool to anchor osteogenic molecules on the surface of implant devices. (AU)

• Processo FAPESP: 10/09113-4 - Influência da nitretação por plasma da superfície do titânio e do colágeno tipo I na adsorção das proteínas séricas sobre a superfície do titânio, e sua influência na adesão, estruturação morfológica e bioatividade de osteoblastos humanos
• Beneficiário: Altair Antoninha Del Bel Cury
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/09308-0 - Influência da nitretação por plasma da superfície do titânio e do colágeno tipo I na adsorção das proteínas séricas sobre a superfície do titânio, e sua influência na adesão, estruturação morfológica e bioatividade de osteoblastos humanos
• Beneficiário: Plinio Mendes Senna
• Modalidade de apoio: Bolsas no Brasil - Doutorado