Probiotic Effects ofLactobacillus paracasei28.4 to InhibitStreptococcus mutansin a Gellan-Based Formulation

Autor(es):Mostrar menos -

de Alvarenga, Janaina Araujo ^[1] ; de Barros, Patricia Pimentel ^[1] ; de Camargo Ribeiro, Felipe ^[1] ; Rossoni, Rodnei Dennis ^[1] ; Garcia, Maira Terra ^[1] ; dos Santos Velloso, Marisol ^[1] ; Shukla, Shashank ^[2] ; Fuchs, Beth Burgwyn ^{[3, 4]} ; Shukla, Anita ^[2] ; Mylonakis, Eleftherios ^{[3, 4]} ; Junqueira, Juliana Campos ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Biosci & Oral Diag, Inst Sci & Technol, Ave Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Brown Univ, Sch Engn, Providence, RI 02912 - USA

^[3] Brown Univ, Providence, RI 02912 - USA

^[4] Rhode Isl Hosp, Alpert Med Sch, Providence, RI - USA

• Tipo de documento: Artigo Científico
• Fonte: PROBIOTICS AND ANTIMICROBIAL PROTEINS; v. 13, n. 2, p. 506-517, APR 2021.
• Citações Web of Science: 0

Resumo

Streptococcus mutansis considered to be a major bacterium involved in dental caries, and the control of virulence mechanisms is fundamental to prevent disease. Probiotics present a promising preventive method; however, the use of probiotics requires its incorporation into delivery materials to facilitate oral colonization. Thus, we performed a comprehensive study examining preventive effects ofLactobacillus paracasei28.4-enriched gellan hydrogel materials to inhibitS.mutansin planktonic and biofilm states, addressing its influence in the production of extracellular polysaccharides (EPS) and altered gene expression of several cariogenic virulence factors.L.paracasei28.4, a strain isolated from the oral cavity of a caries-free individual, was incorporated in three gellan hydrogels (0.5%, 0.75%, and 1% w/v). The pretreatment with probiotic-gellan formulations provided a release ofL.paracaseicells over 24 h that was sufficient to inhibit the planktonic growth ofS.mutans, independent of the gellan concentrations and pH variations. This pretreatment also had inhibitory activity againstS.mutansbiofilms, exhibiting a reduction of 0.57 to 1.54 log(10)in CFU/mL (p < 0.0001) and a decrease of 68.8 to 71.3% in total biomass (p < 0.0001) compared with the control group. These inhibitory effects were associated with the decreased production of EPS by 80% (p < 0.0001) and the downregulation ofluxS,brpA,gbpB, andgtfBgenes. The gellan formulation containingL.paracasei28.4 exhibited probiotic effects for preventingS.mutansgrowth, biofilm formation, and production of cariogenic factors to suggest possible use in tooth decay prevention. (AU)

• Processo FAPESP: 16/25544-1 - Desenvolvimento de formulações probióticas para a prevenção da candidose bucal: Estudo in vitro e in vivo
• Beneficiário: Felipe de Camargo Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/15529-8 - Efeitos de diferentes formulações probióticas contendo Lactobacillus paracasei 28.4 sobre a atividade antibacteriana, produção de polissacarídeos e formação de biofilmes por Streptococcus mutans
• Beneficiário: Janaina Araújo de Alvarenga
• Modalidade de apoio: Bolsas no Brasil - Mestrado