Xylella fastidiosasubsp.paucaStrains Fb7 and 9a5c from Citrus Display Differential Behavior, Secretome, and Plant Virulence

Autor(es)

de Souza, Jessica Brito ^[1] ; Almeida-Souza, Hebreia Oliveira ^[1] ; Zaini, Paulo Adriano ^[2] ; Alves, Monica Neli ^{[3, 4]} ; de Souza, Aline Gomes ^[1] ; Pierry, Paulo Marques ^[5] ; da Silva, Aline Maria ^[5] ; Goulart, Luiz Ricardo ^[1] ; Dandekar, Abhaya M. ^[2] ; Nascimento, Rafael ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Uberlandia, Inst Biotechnol, Ave Amazonas, Bloco 2E, Campus Umuarama, BR-38400902 Uberlandia, MG - Brazil

^[2] Univ Calif Davis, Coll Agr & Environm Sci, Dept Plant Sci, 1 Shields Ave, Davis, CA 95616 - USA

^[3] Sao Paulo State Univ, FCAV UNESP, Sch Agr & Vet Studies, Dept Technol, Via Acesso Prof Paulo Donato Castellane, BR-14884900 Jaboticabal, SP - Brazil

^[4] Citriculture Def Fund Fundecitrus, Ave Dr Adhemar Pereira de Barros 201, BR-14807040 Araraquara, SP - Brazil

^[5] Univ Sao Paulo, Inst Chem, Dept Biochem, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 18 SEP 2020.
• Citações Web of Science: 0

Resumo

Xylella fastidiosacolonizes the xylem of various cultivated and native plants worldwide. Citrus production in Brazil has been seriously affected, and major commercial varieties remain susceptible to Citrus Variegated Chlorosis (CVC). Collective cellular behaviors such as biofilm formation influence virulence and insect transmission ofX. fastidiosa. The reference strain 9a5c produces a robust biofilm compared to Fb7 that remains mostly planktonic, and both were isolated from symptomatic citrus trees. This work deepens our understanding of these distinct behaviors at the molecular level, by comparing the cellular and secreted proteomes of these two CVC strains. Out of 1017 identified proteins, 128 showed differential abundance between the two strains. Different protein families were represented such as proteases, hemolysin-like proteins, and lipase/esterases, among others. Here we show that the lipase/esterase LesA is among the most abundant secreted proteins of CVC strains as well, and demonstrate its functionality by complementary activity assays. More severe symptoms were observed inNicotiana tabacuminoculated with strain Fb7 compared to 9a5c. Our results support that systemic symptom development can be accelerated by strains that invest less in biofilm formation and more in plant colonization. This has potential application in modulating the bacterial-plant interaction and reducing disease severity. (AU)

• Processo FAPESP: 11/24091-0 - Análise do transcriptoma de cepas de Xylella Fastidiosa isoladas de diferentes hospedeiros e busca por RNAs regulatórios
• Beneficiário: Paulo Marques Pierry
• Modalidade de apoio: Bolsas no Brasil - Doutorado