The Quorum Sensing Auto-Inducer 2 (AI-2) Stimulates Nitrogen Fixation and Favors Ethanol Production over Biomass Accumulation in Zymomonas mobilis

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Alencar, Valquiria Campos ^{[1, 2]} ; Silva, Juliana de Fatima dos Santos ^[1] ; Vilas Boas, Renata Ozelami ^[2] ; Farnezio, Vinicius Manganaro ^[1] ; de Maria, Yara N. L. F. ^[2] ; Aciole Barbosa, David ^[2] ; Almeida, Alex Tramontin ^[3] ; de Souza, Emanuel Maltempi ^[3] ; Muller-Santos, Marcelo ^[3] ; Jabes, Daniela L. ^[2] ; Menegidio, Fabiano B. ^[2] ; Costa de Oliveira, Regina ^[2] ; Rodrigues, Tiago ^[1] ; Tersariol, Ivarne Luis dos Santos ^[4] ; Walmsley, Adrian R. ^[5] ; Nunes, Luiz R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas, Alameda Univ S-N, BR-09606045 Sao Bernardo Do Campo, SP - Brazil

^[2] Univ Mogi Cruzes UMC, Nucleo Integrado Biotecnol, Av Dr Candido Xavier Almeida Souza 200, BR-08780911 Mogi Das Cruzes, SP - Brazil

^[3] Univ Fed Parana UFPR, Setor Ciencias Biol, Dept Bioquim & Biol Mol, Rua Cel Francisco H Santos 100, BR-81531980 Curitiba, Parana - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Dept Bioquim, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP - Brazil

^[5] Univ Durham, Dept Biosci, South Rd, Durham DH1 3LE - England

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 11 JUN 2021.
• Citações Web of Science: 1

Resumo

Autoinducer 2 (or AI-2) is one of the molecules used by bacteria to trigger the Quorum Sensing (QS) response, which activates expression of genes involved in a series of alternative mechanisms, when cells reach high population densities (including bioluminescence, motility, biofilm formation, stress resistance, and production of public goods, or pathogenicity factors, among others). Contrary to most autoinducers, AI-2 can induce QS responses in both Gram-negative and Gram-positive bacteria, and has been suggested to constitute a trans-specific system of bacterial communication, capable of affecting even bacteria that cannot produce this autoinducer. In this work, we demonstrate that the ethanologenic Gram-negative bacterium Zymomonas mobilis (a non-AI-2 producer) responds to exogenous AI-2 by modulating expression of genes involved in mechanisms typically associated with QS in other bacteria, such as motility, DNA repair, and nitrogen fixation. Interestingly, the metabolism of AI-2-induced Z. mobilis cells seems to favor ethanol production over biomass accumulation, probably as an adaptation to the high-energy demand of N-2 fixation. This opens the possibility of employing AI-2 during the industrial production of second-generation ethanol, as a way to boost N-2 fixation by these bacteria, which could reduce costs associated with the use of nitrogen-based fertilizers, without compromising ethanol production in industrial plants. (AU)

• Processo FAPESP: 17/08112-3 - Análise da composição e da influência exercida pelo microbioma intestinal de camundongos durante o desenvolvimento de caquexia induzida por transplante de células de câncer pulmonar (LLC)
• Beneficiário: Daniela Leite Jabes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/13197-8 - Caracterização de elementos promotores responsivos ao auto-indutor de quorum sensing (QS) tipo 2 (AI-2) em Zymomonas mobilis
• Beneficiário: Luiz Roberto Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular