Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares

Autor(es)

de Oliveira Tongu, Eriky Akio ^[1] ; Segabinazzi, Lorenzo G. T. M. ^{[1, 2]} ; Alvarenga, Marina Landim ^[1] ; Monteiro, Aldine ^[1] ; Papa, Frederico Ozanam ^[1] ; Alvarenga, Marco Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Vet Med & Anim Sci, Dept Vet Surg & Anim Reprod, UNESP, FMVZ, Botucatu, SP - Brazil

^[2] Ross Univ, Sch Vet Med, POB 334, Basseterre - St Kitts & Nevi

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 169, p. 1-8, JUL 15 2021.
• Citações Web of Science: 0

Resumo

This study aimed to evaluate the effects of mesenchymal stem cell-conditioned medium (MSC-CM) on sperm parameters, intrauterine polymorphonuclear neutrophils (PMN), intrauterine fluid accumulation (IUF), and fertility in mares. In experiment 1, two ejaculates from ten stallions were extended to 50 million sperm/mL using a milk-based extender. Thereafter, 20 mL of extended semen was added of MSC-CM as follows: 0, 5, 10, 15, and 20 mL. Sperm kinetics and plasma membrane integrity were evaluated immediately after dilution (T0) and 2 h post-incubation at 37 degrees C (T2). In experiment 2, mares characterized as resistant (n = 13) or susceptible (n = 7) to endometritis were inseminated with fresh semen 24 h post-induction of ovulation in two (Control and CM-1) and three (Control, CM-1, and CM-2) cycles in a crossover, as follows: control, no pharmacological interference; CM-1, supplementation of semen insemination dose at 3:4 (v:v, MSC-CM:semen); CM-2, 30 mL of MSC-CM was infused into the uterus 24 h before insemination. Endometrial cytology and uterine fluid were collected 6 and 24 h after insemination to evaluate the number of PMNs and concentrations of interleukins IL6, IL10, and TNF alpha. IUF was determined by ultrasonography 24 and 48 h after insemination. Pregnancy status was diagnosed 14 days after ovulation. The addition of MSC-CM to semen did not influence sperm parameters at T0 and T2 (P > 0.05) and reduced (CM-1; P < 0.05) the number of PMNs at 6 h post-insemination in resistant mares. In susceptible mares, PMNs at 6 and 24 h post-insemination, as well as IUF were reduced (P < 0.05) in both treated cycles (CM-1 and CM-2). In addition, MSC-CM downregulated IL6 and upregulated IL10 concentrations in the uterus of susceptible mares after insemination. There were no differences in fertility rates among groups both in resistant (Control, 77%, 10/13; CM-1, 62%, 8/13) and susceptible mares (Control, 42.8%, 3/7; CM-1, 57.1%, 4/7; CM-2, 85.7%. 6/7). In conclusion, MSC-CM did not affect sperm parameters when mixed with diluted semen, and reduced post-insemination inflammatory responses in mares. (C) 2021 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 18/02856-3 - Aspectos clínicos, moleculares e proteômica do útero de éguas com endometrite tratadas com terapias biológicas: plasma rico em plaquetas e meio condicionado por células tronco; e firocoxib.
• Beneficiário: Marco Antonio Alvarenga
• Modalidade de apoio: Auxílio à Pesquisa - Regular