Effects of protein malnutrition on hematopoietic regulatory activity of bone marrow mesenchymal stem cells

Autor(es)

Hastreiter, Araceli Aparecida ^[1] ; dos Santos, Guilherme G. ^[1] ; Makiyama, Edson Naoto ^[1] ; Santos, Ed Wilson Cavalcante ^[1] ; Borelli, Primavera ^[1] ; Fock, Ricardo Ambrosio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 93, JUL 2021.
• Citações Web of Science: 0

Resumo

Protein malnutrition causes anemia and leukopenia as it reduces hematopoietic precursors and impairs the production of mediators that regulate hematopoiesis. Hematopoiesis occurs in distinct bone marrow niches that modulate the processes of differentiation, proliferation and self-renewal of hematopoietic stem cells (HSCs). Mesenchymal stem cells (MSCs) contribute to the biochemical composition of bone marrow niches by the secretion of several growth factors and cytokines, and they play an important role in the regulation of HSCs and hematopoietic progenitors. In this study, we investigated the effect of protein malnutrition on the hematopoietic regulatory function of MSCs. C57BL/6NTaq mice were divided into control and protein malnutrition groups, which received, respectively, a normal protein diet (12% casein) and a low protein diet (2% casein). The results showed that protein malnutrition altered the synthesis of SCF, TFG-beta, Angpt-1, CXCL-12, and G-CSF by MSCs. Additionally, MSCs from the protein malnutrition group were not able to maintain the lymphoid, granulocytic and megakaryocytic-erythroid differentiation capacity compared to the MSCs of the control group. In this way, the comprehension of the role of MSCs on the regulation of the hematopoietic cells, in protein malnutrition states, is for the first time showed. Therefore, we infer that hematopoietic alterations caused by protein malnutrition are due to multifactorial alterations and, at least in part, the MSCs' contribution to hematological impairment. (C) 2021 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 18/25813-8 - Efeito da desnutrição proteica sobre a hematopoese: participação das células endoteliais medulares e ação do fator estimulador de colônias granulocíticas (G-CSF)
• Beneficiário: Ricardo Ambrósio Fock
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/06872-2 - Avaliação de aspectos regulatórios da hematopoese em desnutrição proteica-energética experimental: papel da célula endotelial-like derivada da células tronco mesenquimal medular
• Beneficiário: Ricardo Ambrósio Fock
• Modalidade de apoio: Auxílio à Pesquisa - Regular