Differential expression of miRNAs in the presence of B chromosome in the cichlid fish Astatotilapia latifasciata

Autor(es)

Nascimento-Oliveira, Jordana Inacio ^[1] ; Fantinatti, Bruno Evaristo Almeida ^[2] ; Wolf, Ivan Rodrigo ^[1] ; Cardoso, Adauto Lima ^[1] ; Ramos, Erica ^[1] ; Rieder, Nathalie ^[3] ; de Oliveira, Rogerio ^[4] ; Martins, Cesar ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Struct & Funct Biol, BR-18618689 Botucatu, SP - Brazil

^[2] Univ Ninth July UNINOVE, Sch Med, Bauru, SP - Brazil

^[3] Univ Bonn, Fac Math & Nat Sci, Bonn - Germany

^[4] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Biostat Plant Biol Parasitol & Zool, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Genomics; v. 22, n. 1 MAY 12 2021.
• Citações Web of Science: 0

Resumo

BackgroundB chromosomes (Bs) are extra elements observed in diverse eukaryotes, including animals, plants and fungi. Although Bs were first identified a century ago and have been studied in hundreds of species, their biology is still enigmatic. Recent advances in omics and big data technologies are revolutionizing the B biology field. These advances allow analyses of DNA, RNA, proteins and the construction of interactive networks for understanding the B composition and behavior in the cell. Several genes have been detected on the B chromosomes, although the interaction of B sequences and the normal genome remains poorly understood.ResultsWe identified 727 miRNA precursors in the A. latifasciata genome, 66% which were novel predicted sequences that had not been identified before. We were able to report the A. latifasciata-specific miRNAs and common miRNAs identified in other fish species. For the samples carrying the B chromosome (B+), we identified 104 differentially expressed (DE) miRNAs that are down or upregulated compared to samples without B chromosome (B-) (p <0.05). These miRNAs share common targets in the brain, muscle and gonads. These targets were used to construct a protein-protein-miRNA network showing the high interaction between the targets of differentially expressed miRNAs in the B+ chromosome samples. Among the DE-miRNA targets there are protein-coding genes reported for the B chromosome that are present in the protein-protein-miRNA network. Additionally, Gene Ontology (GO) terms related to nuclear matrix organization and response to stimulus are exclusive to DE miRNA targets of B+ samples.ConclusionsThis study is the first to report the connection of B chromosomes and miRNAs in a vertebrate species. We observed that the B chromosome impacts the miRNAs expression in several tissues and these miRNAs target several mRNAs involved with important biological processes. (AU)

• Processo FAPESP: 15/16661-1 - Cromossomos sexuais, cromossomos B e seus enigmas: sistemas modelo para estudos de evolução cromossômica e genômica
• Beneficiário: César Martins
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/25193-7 - Atuação de piwiRNAs no silenciamento de elementos transponíveis na presença do cromossomo B de Astatotilapia latifasciata (Cichlidae)
• Beneficiário: Jordana Inácio Nascimento Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/09320-6 - Análise integrada de expressão diferencial de miRNAs e mRNAs associados a cromossomos B no ciclídeo Astatotilapia latifasciata
• Beneficiário: Jordana Inácio Nascimento Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado