Diffuse reflectance infrared Fourier transform spectroscopy for a qualitative evaluation of plant leaf pigment extraction

Autor(es)

Barreto, Matheus Sampaio Carneiro ^{[1, 2]} ; dos Reis, Josimar Viera ^[1] ; Muraoka, Takashi ^[3] ; Jemo, Martin ^[1] ; Vergutz, Leonardus ^[1] ; Alleoni, Luis Reynaldo F. ^[2]

Afiliação do(s) autor(es)

^[1] Mohammed VI Polytech Univ UM6P, AgroBiosci Program, Ben Guerir - Morocco

^[2] Univ Sao Paulo, Luiz Queiroz Coll Agr ESALQ, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYST; v. 146, n. 11, p. 3440-3448, JUN 7 2021.
• Citações Web of Science: 0

Resumo

The extraction and quantification of leaf pigments are easy, fast, and cheap procedures; on the other hand, diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy associated with chemometrics tools could offer new insights into leaf biochemical composition. We aimed to boost the classic leaf pigment quantification, adding leaf biochemical information derived from DRIFT spectroscopy + principal component analysis, using the same leaf pigment extract produced by the classical quantification method. We performed a dose-response experiment using P as the limiting nutrient, and maize (Zea mays L.) as a plant-test. After 45 d of growth, we evaluated the effects of P fertilization in total maize shoot biomass, P shoot accumulation, leaf pigment quantification by UV-Vis, and the evaluation of biochemical variations by DRIFT spectroscopy analysis associated with a chemometric approach in the same leaf extract used for pigment quantification. P fertilization raised biomass accumulation (similar to 7.4x), P uptake (similar to 2.3x), and total chlorophyll a and b contents (similar to 2.1x). DRIFT spectroscopy analysis of extracted pigments revealed an elevated content of proteins and polysaccharides at high P availability. At low P availability, we found a low efficiency of N metabolism suggested by the accumulation of inorganic N forms. DRIFT spectroscopy applied together with the classic leaf pigment extraction and quantification method is a novel and promising tool for plant nutrition studies as a DRIFT spectroscopy metabolic profile protocol. (AU)

• Processo FAPESP: 14/22262-0 - Geotecnologias no mapeamento digital pedológico detalhado e biblioteca espectral de solos do Brasil: desenvolvimento e aplicações
• Beneficiário: José Alexandre Melo Demattê
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/22058-9 - Adsorção e dessorção de Cd e Zn em solos tropicais altamente fertilizados
• Beneficiário: Matheus Sampaio Carneiro Barreto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/05870-1 - ESPECIAÇÃO E CINÉTICA DE ADSORÇÃO E DESSORÇÃO DE Cd E DE Zn EM SOLOS DE ÁREAS OLERÍCOLAS ALTAMENTE FERTILIZADAS
• Beneficiário: Matheus Sampaio Carneiro Barreto
• Modalidade de apoio: Bolsas no Brasil - Doutorado