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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diffuse reflectance infrared Fourier transform spectroscopy for a qualitative evaluation of plant leaf pigment extraction

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Author(s):
Barreto, Matheus Sampaio Carneiro [1, 2] ; dos Reis, Josimar Viera [1] ; Muraoka, Takashi [3] ; Jemo, Martin [1] ; Vergutz, Leonardus [1] ; Alleoni, Luis Reynaldo F. [2]
Total Authors: 6
Affiliation:
[1] Mohammed VI Polytech Univ UM6P, AgroBiosci Program, Ben Guerir - Morocco
[2] Univ Sao Paulo, Luiz Queiroz Coll Agr ESALQ, Sao Paulo - Brazil
[3] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ANALYST; v. 146, n. 11, p. 3440-3448, JUN 7 2021.
Web of Science Citations: 0
Abstract

The extraction and quantification of leaf pigments are easy, fast, and cheap procedures; on the other hand, diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy associated with chemometrics tools could offer new insights into leaf biochemical composition. We aimed to boost the classic leaf pigment quantification, adding leaf biochemical information derived from DRIFT spectroscopy + principal component analysis, using the same leaf pigment extract produced by the classical quantification method. We performed a dose-response experiment using P as the limiting nutrient, and maize (Zea mays L.) as a plant-test. After 45 d of growth, we evaluated the effects of P fertilization in total maize shoot biomass, P shoot accumulation, leaf pigment quantification by UV-Vis, and the evaluation of biochemical variations by DRIFT spectroscopy analysis associated with a chemometric approach in the same leaf extract used for pigment quantification. P fertilization raised biomass accumulation (similar to 7.4x), P uptake (similar to 2.3x), and total chlorophyll a and b contents (similar to 2.1x). DRIFT spectroscopy analysis of extracted pigments revealed an elevated content of proteins and polysaccharides at high P availability. At low P availability, we found a low efficiency of N metabolism suggested by the accumulation of inorganic N forms. DRIFT spectroscopy applied together with the classic leaf pigment extraction and quantification method is a novel and promising tool for plant nutrition studies as a DRIFT spectroscopy metabolic profile protocol. (AU)

FAPESP's process: 14/22262-0 - Geotechnologies on a detailed digital soil mapping and the Brazilian soil spectral library: development and applications
Grantee:José Alexandre Melo Demattê
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/22058-9 - Adsorption and desorption of Cd and Zn in highly fertilized tropical soils
Grantee:Matheus Sampaio Carneiro Barreto
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 16/05870-1 - SPCIATION AND KINETIC ADSORPTION AND DESORPTION OF Cd AND Zn IN SOILS OF OLERACEUS CROPS HIGHLY FERTILIZER
Grantee:Matheus Sampaio Carneiro Barreto
Support Opportunities: Scholarships in Brazil - Doctorate