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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Sphingomyelinase D Activity in Sicarius tropicus Venom: Toxic Potential and Clues to the Evolution of SMases D in the Sicariidae Family

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Autor(es):
Lopes, Priscila Hess [1] ; Fukushima, Caroline Sayuri [2, 3] ; Shoji, Rosana [1] ; Bertani, Rogerio [3] ; Tambourgi, V, Denise
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] V, Butantan Inst, Immunochem Lab, BR-05503900 Sao Paulo - Brazil
[2] Univ Helsinki, Finnish Museum Nat Hist, Helsinki 00014 - Finland
[3] Butantan Inst, Special Lab Ecol & Evolut, BR-05503900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: TOXINS; v. 13, n. 4 APR 2021.
Citações Web of Science: 0
Resumo

The spider family Sicariidae includes three genera, Hexophthalma, Sicarius and Loxosceles. The three genera share a common characteristic in their venoms: the presence of Sphingomyelinases D (SMase D). SMases D are considered the toxins that cause the main pathological effects of the Loxosceles venom, that is, those responsible for the development of loxoscelism. Some studies have shown that Sicarius spiders have less or undetectable SMase D activity in their venoms, when compared to Hexophthalma. In contrast, our group has shown that Sicarius ornatus, a Brazilian species, has active SMase D and toxic potential to envenomation. However, few species of Sicarius have been characterized for their toxic potential. In order to contribute to a better understanding about the toxicity of Sicarius venoms, the aim of this study was to characterize the toxic properties of male and female venoms from Sicarius tropicus and compare them with that from Loxosceles laeta, one of the most toxic Loxosceles venoms. We show here that S. tropicus venom presents active SMases D. However, regarding hemolysis development, it seems that these toxins in this species present different molecular mechanisms of action than that described for Loxosceles venoms, whereas it is similar to those present in bacteria containing SMase D. Besides, our results also suggest that, in addition to the interspecific differences, intraspecific variations in the venoms' composition may play a role in the toxic potential of venoms from Sicarius species. (AU)

Processo FAPESP: 15/17053-5 - Estudo da ativação de inflamassomas, em queratinócitos humanos, por ação do veneno da aranha Loxosceles laeta e sua esfingomielinase D
Beneficiário:Priscila Hess Lopes
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
Beneficiário:Hugo Aguirre Armelin
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs