Occurrence and diversity of Sarcocystidae protozoa in muscle and brain tissues of bats from Sao Paulo state, Brazil

Autor(es)

Cabral, Aline Diniz ^{[1, 2]} ; Su, Chunlei ^[3] ; Soares, Rodrigo Martins ^[1] ; Gennari, Solange Maria ^{[1, 4, 5]} ; Speranca, Marcia Aparecida ^[2] ; da Rosa, Adriana Ruckert ^[6] ; Pena, Hilda Fatima Jesus ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Ave Prof Dr Orlando Marques de Paiva, 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas CCNH, Rua Arcturus, 03, BR-09606045 Sao Bernardo Do Campo, SP - Brazil

^[3] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 - USA

^[4] Univ Santo Amaro, Programa Posgrad Med, R Prof Eneas de Siqueira Neto, 340, BR-04829300 Sao Paulo, SP - Brazil

^[5] Univ Santo Amaro, Bem Estar Anim & Saude Publ, R Prof Eneas de Siqueira Neto, 340, BR-04829300 Sao Paulo, SP - Brazil

^[6] Lab Zoonoses & Doencas Transmitidas Vetores LabZo, Controle Zoonoses CCZ, Rua Santa Eulalia, 86, BR-02031020 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE; v. 14, p. 91-96, APR 2021.
• Citações Web of Science: 0

Resumo

Studies on infectious and emerging diseases caused by bats have been increasing worldwide due to their well-recognised status as a reservoir species for various infectious agents as well as their close relationship to humans and animals. This study reports the molecular frequency and diversity of the parasites belonging to the Sarcocystidae family in bats in Sao Paulo state, Brazil. A total of 2892 tissue samples (brain and pectoral muscle/heart homogenates) from 1921 bats belonging to 36 species were collected, and the Sarcocystidae protozoan 18S ribosomal RNA encoding genes (18S rDNA) were detected by nested PCR and Sanger sequencing. The relative prevalence of Sarcocystidae species was 4.7% (91/1921) among 16 bat species, including insectivorous (n = 65), frugivorous (n = 13) and nectarivorous (n = 11) bats. From 66 sequenced positive samples, 50 were found to be suitable for analysis. Ten samples from insectivorous and nectarivorous bats showed 100% similarity with Neospora caninum (n = 1), Hammondia hammondi (n = 1), Cystoisospora canis (n = 1), Nephroisospora eptesici (n = 1), Sarcocystis (Frenkelia) glareoli (n = 1), and Toxoplasma gondii (n = 5). The 45 non-T. gondii samples revealed 15 different 18S rDNA alleles with identities varying from 96.1 to 100% with several Sarcocystidae species, which might suggest that bats can harbour a large variety of Sarcocystidae organisms. From the five T. gondii-positive tissue samples, three samples from two different bat specimens of the insectivorous Eumops glacinus were characterised using 11 PCR-restriction fragment length polymorphism (RFLP) markers, revealing the non-archetypal ToxoDB genotypes \#6 (type BrI), which is one of the most prevalent in different hosts and regions from Brazil, and \#69. We recommend the inclusion of T. gondii as a differential diagnosis for rabies and other neurological syndromes in bats. (AU)

• Processo FAPESP: 09/51889-2 - Epidemiologia da Toxoplasma gondii (soroprevalência, isolamento e genotipagem) e investigação molecular de outros protozoários perten centes a sub-família Toxoplasmatinae (Neospora spp e hammondia spp)..
• Beneficiário: Aline Diniz Cabral
• Modalidade de apoio: Bolsas no Brasil - Doutorado