Harmful Effects of Granulocytic Myeloid-Derived Suppressor Cells on Tuberculosis Caused by Hypervirulent Mycobacteria

Autor(es):Mostrar menos -

Barbosa Bomfim, Caio Cesar ^[1] ; Amaral, Eduardo Pinheiro ^[2] ; Santiago-Carvalho, Igor ^[1] ; Santos, Gislane Almeida ^[1] ; Salles, Erika Machado ^[1] ; Hastreiter, Araceli Aparecida ^[3] ; do Nascimento, Rogerio Silva ^[1] ; Almeida, Fabricio M. ^[4] ; Bia Ventura Simao, Thatiana Lopes ^[4] ; Rezende, Andreza Linhares ^[4] ; Hirata, Mario Hiroyuki ^[3] ; Fock, Ricardo Ambrosio ^[3] ; Alvarez, Jose Maria ^[1] ; Lasunskaia, Elena B. ^[4] ; D'Imperio Lima, Maria Regina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo - Brazil

^[2] NIAID, Immunobiol Sect, Lab Parasit Dis, NIH, 9000 Rockville Pike, Bethesda, MD 20892 - USA

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil

^[4] Univ Estadual Norte Fluminense, Lab Biol Reconhecer, Campos Dos Goyiacazes, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Infectious Diseases; v. 223, n. 3, p. 494-507, FEB 1 2021.
• Citações Web of Science: 1

Resumo

Background. The role of myeloid-derived suppressor cells (MDSCs) in patients with severe tuberculosis who suffer from uncontrolled pulmonary inflammation caused by hypervirulent mycobacterial infection remains unclear. Methods. This issue was addressed using C57BL/6 mice infected with highly virulent Mycobacterium bovis strain MP287/03. Results. CD11b(+)GR1(int) population increased in the bone marrow, blood and lungs during advanced disease. Pulmonary CD11b(+)GR1(int) (Ly(6)G(int)Ly6C(int)) cells showed granularity similar to neutrophils and expressed immature myeloid cell markers. These immature neutrophils harbored intracellular bacilli and were preferentially located in the alveoli. T-cell suppression occurred concomitantly with CD11b(+)GR1(int) cell accumulation in the lungs. Furthermore, lung and bone marrow GR1(+) cells suppressed both T-cell proliferation and interferon gamma production in vitro. Anti-GR1 therapy given when MDSCs infiltrated the lungs prevented expansion and fusion of primary pulmonary lesions and the development of intragranulomatous caseous necrosis, along with increased mouse survival and partial recovery of T-cell function. Lung bacterial load was reduced by anti-GR1 treatment, but mycobacteria released from the depleted cells proliferated extracellularly in the alveoli, forming cords and clumps. Conclusions. Granulocytic MDSCs massively infiltrate the lungs during infection with hypervirulent mycobacteria, promoting bacterial growth and the development of inflammatory and necrotic lesions, and are promising targets for host-directed therapies. (AU)

• Processo FAPESP: 15/20432-8 - Intervenção em vias de sinalização associadas ao reconhecimento de dano celular visando reduzir a patologia das formas graves de malária e tuberculose
• Beneficiário: Maria Regina D'Império Lima
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/09110-4 - Caracterização morfológica e funcional de células mieloides imaturas na tuberculose severa.
• Beneficiário: Caio César Barbosa Bomfim
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/22986-8 - Papel das células mielóides supressoras e da sua regulação purinérgica na tuberculose severa
• Beneficiário: Caio César Barbosa Bomfim
• Modalidade de apoio: Bolsas no Brasil - Doutorado