Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia

Autor(es):Mostrar menos -

Valenca, Ian Nunes ^{[1, 2]} ; dos Santos, Rafael Bezerra ^{[1, 2]} ; Peronni, Kamila Chagas ^[2] ; Sauvage, Virginie ^[3] ; Vandenbogaert, Mathias ^[4] ; Caro, Valerie ^[4] ; da Silva Junior, Wilson Araujo ^[5] ; Covas, Dimas Tadeu ^{[6, 2]} ; Silva-Pinto, Ana Cristina ^[2] ; Laperche, Syria ^[3] ; Kashima, Simone ^[2] ; Slavov, Svetoslav Nanev ^{[6, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Programa Mestrado Oncol Clin Celulas Tronco & Ter, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Hemoctr Ribeirao Preto, Rua Tenente Catao Roxo 2501, BR-14051060 Ribeirao Preto, SP - Brazil

^[3] Ctr Natl Reference Risques Infect Transfus, Inst Natl Transfus, Dept Etud Agents Transmissibles Sang, Paris - France

^[4] Inst Pasteur, Unite Environm & Risques Infect, Cellule Intervent Biol Urgence, Paris - France

^[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 63, 2021.
• Citações Web of Science: 0

Resumo

ABSTRACT To date, blood banks apply routine diagnosis to a specific spectrum of transfusion-transmitted viruses. Even though this measure is considered highly efficient to control their transmission, the threat imposed by emerging viruses is increasing globally, which can impact transfusion safety, especially in the light of the accelerated viral discovery by novel sequencing technologies. One of the most important groups of patients, who may indicate the presence of emerging viruses in the field of blood transfusion, is the group of individuals who receive multiple transfusions due to hereditary hemoglobinopathies. It is possible that they harbor unknown or unsuspected parenterally-transmitted viruses. In order to elucidate this, nucleic acids from 30 patients with beta-thalassemia were analyzed by Illumina next-generation sequencing and bioinformatics analysis. Three major viral families: Anelloviridae, Flaviviridae and Hepadnaviridae were identified. Among them, anelloviruses were the most representative, being detected with high number of reads in all tested samples. Human Pegivirus 1 (HPgV-1, or GBV-C), Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) were also identified. HBV and HCV detection was expected due to the high seroprevalence in patients with beta thalassemia. Our results do not confirm the presence of emerging or unsuspected viruses threatening the transfusion safety at present, but can be used to actively search for viruses that threaten blood transfusion safety. We believe that the application of viral metagenomics in multiple-transfused patients is highly useful to monitor possible viral transfusion threats and for the annotation of their virome composition. (AU)

• Processo FAPESP: 19/07861-8 - Desenvolvimento de um pipeline de bioinformática para identificação de doenças infecciosas emergentes em pacientes em regime de transfusão crônica
• Beneficiário: Ian Nunes Valença
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/15826-5 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas
• Beneficiário: Svetoslav Nanev Slavov
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 19/08528-0 - Aplicação de métodos de bioinformática para identificação de infecções virais com impacto em hemoterapia
• Beneficiário: Rafael dos Santos Bezerra
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/23205-8 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas
• Beneficiário: Svetoslav Nanev Slavov
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores