| Texto completo | |
| Autor(es): |
Batissoco, A. C.
[1, 2]
;
Lezirovitz, K.
[1, 2]
;
Zanatta, D. B.
[3]
;
Hemza, C. R. M. L.
[2]
;
Vasques, L. R.
[4]
;
Strauss, B. E.
[3]
;
Mingroni-Netto, R. C.
[4]
;
Haddad, L. A.
[4]
;
Bento, R. F.
[1, 2]
;
Oiticica, J.
[1, 2]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Hosp Clin, Fac Med, 0Torrinolaringol LIM32, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Dept Otorrinolaringol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Med, Ctr Invest Translac Oncol LIM24, Inst Canc Estado Sao Paulo, Lab Vetores Virais, Sao Paulo - Brazil
[4] Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Medical and Biological Research; v. 54, n. 7 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
NOTCH pathway proteins, including the transcriptional factor HES1, play crucial roles in the development of the inner ear by means of the lateral inhibition mechanism, in which supporting cells have their phenotype preserved while they are prevented from becoming hair cells. Genetic manipulation of this pathway has been demonstrated to increase hair cell number. The present study aimed to investigate gene expression effects in hair cells and supporting cells after Hes1-shRNA lentivirus transduction in organotypic cultures of the organ of Corti from postnatal-day-3 mice. Forty-eight hours after in vitro knockdown, Hes1 gene expression was reduced at both mRNA and protein levels. Myo7a (hair cell marker) and Sox2 (progenitor cell marker) mRNA levels also significantly increased. The modulation of gene expression in the organ of Corti upon Hes1 knockdown is consistent with cell phenotypes related to lateral inhibition mechanism interference in the inner ear. The lentivirus-based expression of Hes1-shRNA is a valuable strategy for genetic interference in the organ of Corti and for future evaluation of its efficacy in protocols aiming at the regeneration of hair cells in vivo. (AU) | |
| Processo FAPESP: | 09/09473-3 - Sinalização e diferenciação das células do órgão de Corti: perspectivas para a terapia da surdez |
| Beneficiário: | Ricardo Ferreira Bento |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/08028-1 - CEGH-CEL - Centro de Estudos do Genoma Humano e de Células-Tronco |
| Beneficiário: | Mayana Zatz |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 08/00458-9 - Avaliação da regeneração do nervo facial de ratos após a implantação de células tronco derivadas do estroma de medula óssea diferenciadas in vitro |
| Beneficiário: | Ricardo Ferreira Bento |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |