| Full text | |
| Author(s): |
Batissoco, A. C.
[1, 2]
;
Lezirovitz, K.
[1, 2]
;
Zanatta, D. B.
[3]
;
Hemza, C. R. M. L.
[2]
;
Vasques, L. R.
[4]
;
Strauss, B. E.
[3]
;
Mingroni-Netto, R. C.
[4]
;
Haddad, L. A.
[4]
;
Bento, R. F.
[1, 2]
;
Oiticica, J.
[1, 2]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Hosp Clin, Fac Med, 0Torrinolaringol LIM32, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Dept Otorrinolaringol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Med, Ctr Invest Translac Oncol LIM24, Inst Canc Estado Sao Paulo, Lab Vetores Virais, Sao Paulo - Brazil
[4] Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Medical and Biological Research; v. 54, n. 7 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
NOTCH pathway proteins, including the transcriptional factor HES1, play crucial roles in the development of the inner ear by means of the lateral inhibition mechanism, in which supporting cells have their phenotype preserved while they are prevented from becoming hair cells. Genetic manipulation of this pathway has been demonstrated to increase hair cell number. The present study aimed to investigate gene expression effects in hair cells and supporting cells after Hes1-shRNA lentivirus transduction in organotypic cultures of the organ of Corti from postnatal-day-3 mice. Forty-eight hours after in vitro knockdown, Hes1 gene expression was reduced at both mRNA and protein levels. Myo7a (hair cell marker) and Sox2 (progenitor cell marker) mRNA levels also significantly increased. The modulation of gene expression in the organ of Corti upon Hes1 knockdown is consistent with cell phenotypes related to lateral inhibition mechanism interference in the inner ear. The lentivirus-based expression of Hes1-shRNA is a valuable strategy for genetic interference in the organ of Corti and for future evaluation of its efficacy in protocols aiming at the regeneration of hair cells in vivo. (AU) | |
| FAPESP's process: | 09/09473-3 - Signaling and differentiation of the organ of Corti cells: perspectives for treating hearing loss |
| Grantee: | Ricardo Ferreira Bento |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center |
| Grantee: | Mayana Zatz |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 08/00458-9 - Evaluation of the facial nerve regeneration in rat after vitro differentiation bone marrow stromal cells implantation |
| Grantee: | Ricardo Ferreira Bento |
| Support Opportunities: | Regular Research Grants |