Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice

Autor(es)

Santos, Geovane Ribeiro ^{[1, 2, 3]} ; Cunha, Marcelo Rodrigues ^[1] ; Caldeira, Eduardo Jose ^[1] ; Galdeano, Ewerton Alexandre ^[1] ; Seabra Prudente, Raphael Cruz ^[4] ; Lopes Pinto, Clovis Antonio ^{[1, 2, 5]}

Afiliação do(s) autor(es)

^[1] Jundiai Med Sch JMS Jundiai, Morphol & Basic Pathol Dept, Francisco Telles 250, Vila Arens 2 1109, BR-13202550 Jundiai, SP - Brazil

^[2] Jundiai Med Sch JMS Jundiai, Pathol & Cytol Lab, Jundiai, SP - Brazil

^[3] Univ Paulista, ICS, Inst Hlth Sci, UNIP Campus Jundiai, Jundiai, SP - Brazil

^[4] Hlth Management & Promot Unit HMPU, Jundiai, SP - Brazil

^[5] Hosp AC Camargo Fund Antonio Prudente, Pathol Anat Serv, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 JUN 7 2021.
• Citações Web of Science: 0

Resumo

The sebaceous gland (SG) is involved in different inflammatory, infectious and neoplastic processes of the skin and can be related to specific diseases, e.g., diabetes mellitus. Sometimes, the histological diagnosis requires complementary tests due to the ability of diseases to mimic other tumors. We evaluated the sebaceous gland density in Non-obese diabetic mice to analyze the N-acetylcystein effects and swimming exercise treatment in sebaceous glands healing, using specific staining in histochemistry and immunohistochemistry reactions in the identification of the lipid expression in the sebaceous gland. We investigated the intracytoplasmic lipid expression and analysis of gland density from SG in dorsal skin samples from the Non-obese diabetic (NOD mice) and diabetic animals submitted to antioxidant treatment and physical exercise. For histological analysis of the sebaceous glands, specific staining in histochemistry with sudan black and immunohistochemistry reaction with adipophilin were used in the evaluation. Statistical analysis showed significant proximity between the values of the control group and the diabetic group submitted to the swimming exercise (DS group) and similar values between the untreated diabetic group (UD group) and diabetic group treated with the antioxidant N-acetylcysteine (DNa group), which did not prevent possible differences where p<0.01. Adipophilin (ADPH) immunohistochemistry permitted more intense lipid staining in SGs, the preservation of the SG in the control group, and a morphological deformed appearance in the UD and DNa groups. However, weak morphological recovery of the SG was observed in the DS-Na group, being more expressive in the DS group. In conclusion, the groups submitted to physical exercises showed better results in the recovery of the analyzed tissue, even being in the physiological conditions caused by spontaneous diabetes. (AU)

• Processo FAPESP: 12/18012-2 - Papel dos peptídeos p217 e p290 na ativação de ins-r em glândulas salivares de camundongos diabéticos
• Beneficiário: Eduardo José Caldeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular