Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis

Autor(es):Mostrar menos -

Balthazar, Celso F. ^{[1, 2]} ; de Moura, Nelci A. ^[3] ; Romualdo, Guilherme R. ^[3] ; Rocha, Ramon S. ^{[2, 4]} ; Pimentel, Tatiana C. ^[5] ; Esmerino, Erick A. ^{[2, 4, 6]} ; Freitas, Monica Q. ^[2] ; Santillo, Antonella ^[7] ; Silva, Marcia Cristina ^[4] ; Barbisan, Luis F. ^[2] ; Cruz, Adriano G. ^[4] ; Albenzio, Marzia ^[7]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci & Nutr, BR-13083862 Campinas, SP - Brazil

^[2] Fed Fluminense Univ UFF, Vet Sch, Dept Vet Hyg & Technol Proc Anim Prod, BR-24220000 Niteroi, RJ - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Struct & Funct Biol, BR-18618970 Botucatu, SP - Brazil

^[4] Fed Inst Rio de Janeiro IFRJ, Dept Food, BR-20270021 Rio De Janeiro, RJ - Brazil

^[5] Fed Inst Parana IFPR, Dept Food Sci, BR-87703536 Paranavai, Parana - Brazil

^[6] Rural Fed Univ Rio de Janeiro UFRRJ, Dept Food Technol, BR-23890000 Seropedica, RJ - Brazil

^[7] Univ Foggia UNIFG, Dept Agr Food Nat Resources & Engn DAFNE, I-71122 Foggia - Italy

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DAIRY SCIENCE; v. 104, n. 7, p. 7406-7414, JUL 2021.
• Citações Web of Science: 0

Resumo

Colorectal cancer (CRC) is one of the most common human cancers mainly in Western industrialized countries (Bray et al., 2018; Siegel et al., 2019). Globally, CRC is the third most commonly diagnosed malignancy and the second leading cause of cancer death (Keum and Giovannucci, 2019). In Brazil, it is estimated that 16,630 men and 19,030 women were diagnosed with CRC in 2020 (INCA, 2020). Epidemiological and ex Sheep dairy products containing prebiotic and pro biotic ingredients may have health-promoting properties. Thus, this study evaluated the effects of sheep milk ice cream {[}conventional full-fat (CONV), full-fat enriched with probiotic (PROB, 100 mg % wt/wt of Lacticaseibacillus casei 01), or nonfat synbiotic (SYNB, Lacticaseibacillus casei 01 and inulin, 10% wt/wt)] on carcinogen-induced colonic crypt cytotoxicity and premalignant lesion development. Male Swiss mice received 2 doses of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at wk 3 and 4. Two weeks before and during AOM administrations (4 wk) mice were treated with CONV, PROB, or SYNB by gavage (10 mL/kg). Mice were euthanized at wk 4 or 25 (n = 5 or 10 mice/group, respectively). At wk 4, a significant reduction in micronucleated colonocytes was observed in PROB and SYNB groups, and a significant decrease in both p53 expression and apoptosis indexes in colonic crypts was observed in SYNB group. At wk 25, both PROB and SYNB interventions reduced the mean number of colonic premalignant lesions. However, only SYNB group showed lower incidence and number of high-grade premalignant lesions in the colonic mucosa. These findings indicate that PROB or SYNB sheep milk ice cream, especially SYNB intervention, can reduce chemically induced mouse colon carcinogenesis. (AU)

• Processo FAPESP: 18/24540-8 - Leite de ovelha processado por aquecimento ôhmico para produção de iogurte: inativação de patógenos e estudo da ecologia microbiana durante a vida útil por sequenciamento de amplicon do gene 16S rRNA
• Beneficiário: Celso Fasura Balthazar
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado