Oxidative phenotype induced by aerobic physical training prevents the obesity-linked insulin resistance without changes in gastrocnemius muscle ACE2-Angiotensin(1-7)-Mas axis

Autor(es)

Vecchiatto, Bruno ^[1] ; da Silva, Rafael C. ^[1] ; Higa, Talita S. ^[1] ; Muller, Cynthia R. ^[2] ; Americo, V, Anna Laura ; Fortunato-Lima, Vanessa C. ^[1] ; Ferreira, Marilia M. ^[1] ; Martucci, Luiz Felipe ^[3] ; Fonseca-Alaniz, Miriam H. ^[4] ; Evangelista, Fabiana S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Arts Sci & Humanities, Av Arlindo Bettio 1000, BR-03828000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Expt Pathophysiol, Sao Paulo - Brazil

^[3] Americo, Anna Laura, V, Univ Sao Paulo, Fac Med, Dept Expt Pathophysiol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med, Heart Inst InCor, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DIABETOLOGY & METABOLIC SYNDROME; v. 13, n. 1 JUL 6 2021.
• Citações Web of Science: 0

Resumo

Background We investigate the effect of aerobic physical training (APT) on muscle morphofunctional markers and Angiotensin Converting Enzyme 2/Angiotensin 1-7/Mas receptor (ACE2/Ang 1-7/Mas) axis in an obesity-linked insulin resistance (IR) animal model induced by cafeteria diet (CAF). Methods Male C57BL/6J mice were assigned into groups CHOW-SED (chow diet, sedentary; n = 10), CHOW-TR (chow diet, trained; n = 10), CAF-SED (n = 10) and CAF-TR (n = 10). APT consisted in running sessions of 60 min at 60% of maximal speed, 5 days per week for 8 weeks. Results Trained groups had lower body weight and adiposity compared with sedentary groups. CAF-TR improved the glucose and insulin tolerance tests compared with CAF-SED group (AUC = 28.896 +/- 1589 vs. 35.200 +/- 1076 mg dL(-1) 120 min(-1); kITT = 4.1 +/- 0.27 vs. 2.5 +/- 0.28% min(-1), respectively). CHOW-TR and CAF-TR groups increased exercise tolerance, running intensity at which VO2 max was reached, the expression of p-AMPK, p-ACC and PGC1-alpha proteins compared with CHOW-SED and CAF-SED. Mithocondrial protein expression of Mfn1, Mfn2 and Drp1 did not change. Lipid deposition reduced in CAF-TR compared with CAF-SED group (3.71 vs. 5.53%/area), but fiber typing, glycogen content, ACE2 activity, Ang 1-7 concentration and Mas receptor expression did not change. Conclusions The APT prevents obesity-linked IR by modifying the skeletal muscle phenotype to one more oxidative independent of changes in the muscle ACE2/Ang 1-7/Mas axis. (AU)

• Processo FAPESP: 16/20659-5 - Papel da alça ECA2/ANG 1-7/Mas no músculo esquelético para a prevenção de distúrbios metabólicos através do treinamento físio aeróbio
• Beneficiário: Bruno Vecchiatto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/04948-4 - Papel da alça ECA2/Ang 1-7/Mas na prevenção da obesidade e da resistência à insulina através do treinamento físico
• Beneficiário: Fabiana de Sant'Anna Evangelista
• Modalidade de apoio: Auxílio à Pesquisa - Regular