| Full text | |
| Author(s): |
Vecchiatto, Bruno
[1]
;
da Silva, Rafael C.
[1]
;
Higa, Talita S.
[1]
;
Muller, Cynthia R.
[2]
;
Americo, V, Anna Laura
;
Fortunato-Lima, Vanessa C.
[1]
;
Ferreira, Marilia M.
[1]
;
Martucci, Luiz Felipe
[3]
;
Fonseca-Alaniz, Miriam H.
[4]
;
Evangelista, Fabiana S.
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Sch Arts Sci & Humanities, Av Arlindo Bettio 1000, BR-03828000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Dept Expt Pathophysiol, Sao Paulo - Brazil
[3] Americo, Anna Laura, V, Univ Sao Paulo, Fac Med, Dept Expt Pathophysiol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Med, Heart Inst InCor, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | DIABETOLOGY & METABOLIC SYNDROME; v. 13, n. 1 JUL 6 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Background We investigate the effect of aerobic physical training (APT) on muscle morphofunctional markers and Angiotensin Converting Enzyme 2/Angiotensin 1-7/Mas receptor (ACE2/Ang 1-7/Mas) axis in an obesity-linked insulin resistance (IR) animal model induced by cafeteria diet (CAF). Methods Male C57BL/6J mice were assigned into groups CHOW-SED (chow diet, sedentary; n = 10), CHOW-TR (chow diet, trained; n = 10), CAF-SED (n = 10) and CAF-TR (n = 10). APT consisted in running sessions of 60 min at 60% of maximal speed, 5 days per week for 8 weeks. Results Trained groups had lower body weight and adiposity compared with sedentary groups. CAF-TR improved the glucose and insulin tolerance tests compared with CAF-SED group (AUC = 28.896 +/- 1589 vs. 35.200 +/- 1076 mg dL(-1) 120 min(-1); kITT = 4.1 +/- 0.27 vs. 2.5 +/- 0.28% min(-1), respectively). CHOW-TR and CAF-TR groups increased exercise tolerance, running intensity at which VO2 max was reached, the expression of p-AMPK, p-ACC and PGC1-alpha proteins compared with CHOW-SED and CAF-SED. Mithocondrial protein expression of Mfn1, Mfn2 and Drp1 did not change. Lipid deposition reduced in CAF-TR compared with CAF-SED group (3.71 vs. 5.53%/area), but fiber typing, glycogen content, ACE2 activity, Ang 1-7 concentration and Mas receptor expression did not change. Conclusions The APT prevents obesity-linked IR by modifying the skeletal muscle phenotype to one more oxidative independent of changes in the muscle ACE2/Ang 1-7/Mas axis. (AU) | |
| FAPESP's process: | 16/20659-5 - The role of ACE2/Ang-(1-7)/Mas axis on skeletal muscle for prevention of metabolic disorders by aerobic training |
| Grantee: | Bruno Vecchiatto |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 15/04948-4 - The role of ECA2/Ang 1-7/Mas axis in the prevention of obesity and insulin resistance by physical training |
| Grantee: | Fabiana de Sant'Anna Evangelista |
| Support Opportunities: | Regular Research Grants |