Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses

Autor(es)

Mirandola Dias Vieira, Diego Henrique ^[1] ; Narciso, Rodrigo Bravin ^[1] ; de Azevedo, Rodney Kozlowiski ^[2] ; da Silva, Reinaldo Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Biosci, Div Parasitol, Lab Parasitol Wild Anim, UNESP, R Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

^[2] Ctr Univ CESMAC, Programa Posgrad Anal Sistemas Ambientais, Maceio, Alagoas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA PARASITOLOGICA; JUL 2021.
• Citações Web of Science: 0

Resumo

Purpose We describe two new Henneguya spp. (Myxobolidae) found parasitizing Cyphocharax modestus from Pardo river, Parana river basin, municipality of Botucatu, Sao Paulo State, Brazil: Henneguya fastigata n. sp. from gill lamellae and Henneguya pardensis n. sp. from gill arches. We based the descriptions on myxospore morphology, histology, and small subunit ribosomal DNA sequences. Methods In this investigation, 50 specimens of Cyphocharax modestus were examined between July and December 2020 for myxozoan infections. Morphological characterization was based on the mature myxospores. The small subunit ribosomal DNA sequences were used for molecular and phylogenetic study. Results Phylogenetic analysis provided the evolutionary position of these new species with other myxobolids species. Myxospores of the two species had similar morphology and morphometrics, but differed in spore body width and length, and ssrDNA sequences differed by 7.2%. These data supported the diagnosis of the parasites as distinct and novel species. The phylogenetic analysis showed a well-supported subclade formed by species that parasitize curimatid fishes, with Henneguya gilbert as a sister species of Henneguya fastigata n. sp., and Henneguya pardensis n. sp. as a sister species of both species. Conclusion Our analysis was consistent with previous studies suggesting that orders and families of the hosts are strongly correlated with phylogenetic signals in the Myxobolidae. These are the first species of myxozoans described in the Pardo river. (AU)

• Processo FAPESP: 19/26831-2 - Diversidade e sistemática de ergasílideos parasitas de peixes do Rio Pardo, Médio Rio Paranapanema, São Paulo
• Beneficiário: Rodrigo Bravin Narciso
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 20/05412-9 - Diversidade, taxonomia e sistemática de parasitos de peixes do Rio Pardo, Médio Rio Paranapanema, São Paulo
• Beneficiário: Reinaldo José da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 19/19060-0 - Sistemática e distribuição de mixozoários coletados em peixes oriundos do Rio Pardo, médio Rio Paranapanema, São Paulo
• Beneficiário: Diego Henrique Mirandola Dias Vieira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado