Signaling Biomaterial for Dental Pulp Regeneration

Autor(es)

Leite, Maria Luisa ^[1] ; Soares, Diana Gabriela ^[2] ; Anovazzi, Giovana ^[3] ; Anselmi, Caroline ^[3] ; Hebling, Josimeri ^[3] ; de Souza Costa, Carlos Alberto ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 47, n. 7, p. 1110-1117, JUL 2021.
• Citações Web of Science: 1

Resumo

Introduction: Guided tissue regeneration has been considered a promising biological strategy to replace conventional endodontic therapies of teeth with incomplete root formation. Therefore, in the present study, a collagen/gelatin hydrogel either containing dosages of fibronectin (FN), or not, was developed and assessed concerning their bioactive and chemotactic potential on human apical papilla cells (hAPCs). Methods: Hydrogels were prepared by varying the ratio of collagen and gelatin (Col/Gel; v/v), and used to establish the following groups: Collagen (positive control); Col/Gel 4:6; Col/Gel 6:4; Col/Gel 8:2. The viability, adhesion, and spreading of cells seeded on the hydrogels were evaluated. Different concentrations of FN (0, 5, or 10 mg/mL) were incorporated into the best formulation of the collagen/gelatin hydrogel selected. Then, the hAPCs seeded on the biomaterials were assessed concerning the cell migration, viability, adhesion and spreading, and gene expression of ITGA5, ITGAV, COL1A1, and COL3A1. Results: The Col/Gel 8:2 group exhibited better cell viability, adhesion and spreading in comparison with Control. Higher values of hAPC migration, viability, adhesion, spreading and gene expression of pulp regeneration markers were found, the higher the concentration was of FN incorporated into the collagen/ gelatin hydrogel. Conclusion: Collagen/gelatin hydrogel with 10 mg/mL of FN had potent bioactive and chemotactic effects on cultured hAPCs. (J Endod 2021;47:1110-1117.) (AU)

• Processo FAPESP: 17/14210-8 - Síntese e avaliação de biomateriais associados a proteínas da matriz extracelular para a regeneração do tecido pulpar
• Beneficiário: Maria Luísa de Alencar e Silva Leite
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/22739-9 - Desenvolvimento e análise de um scaffold bicamada para regeneração do complexo dentino-pulpar
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular