Microfluidic encapsulation of nanoparticles in alginate microgels gelled via competitive ligand exchange crosslinking

Autor(es)

Cinel, Victor Dal Posolo ^[1] ; Taketa, Thiago Bezerra ^[2] ; de Carvalho, Bruna Gregatti ^[2] ; de la Torre, Lucimara Gaziola ^[2] ; de Mello, Lucas Rodrigues ^[1] ; da Silva, Emerson Rodrigo ^[1] ; Han, Sang Won ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biophys, 745 Botucatu St, BR-04023062 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Dept Bioproc & Mat Engn, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biopolymers; v. 112, n. 7 JUL 2021.
• Citações Web of Science: 0

Resumo

Efficient delivery of nanometric vectors complexed with nanoparticles at a target tissue without spreading to other tissues is one of the main challenges in gene therapy. One means to overcome this problem is to confine such vectors within microgels that can be placed in a target tissue to be released slowly and locally. Herein, a conventional optical microscope coupled to a common smartphone was employed to monitor the microfluidic production of monodisperse alginate microgels containing nanoparticles as a model for the encapsulation of vectors. Alginate microgels (1.2%) exhibited an average diameter of 125 +/- 3 mu m, which decreased to 106 +/- 5 mu m after encapsulating 30 nm fluorescent nanoparticles. The encapsulation efficiency was 70.9 +/- 18.9%. In a 0.1 M NaCl solution, 55 +/- 5% and 92 +/- 4.7% of nanoparticles were released in 30 minutes and 48 hours, respectively. Microgel topography assessment by atomic force microscopy revealed that incorporation of nanoparticles into the alginate matrix changes the scaffold's interfacial morphology and induces crystallization with the appearance of oriented domains. The high encapsulation rate of nanoparticles, alongside their continuous release of nanoparticles over time, makes these microgels and the production unit a valuable system for vector encapsulation for gene therapy research. (AU)

• Processo FAPESP: 18/19537-8 - Microfluídica como plataforma tecnológica para nano & biotecnologia
• Beneficiário: Lucimara Gaziola de la Torre
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/18523-3 - Síntese de micropartículas poliméricas via processo microfluídico de gotas para a liberação sustentada de vetores não virais aplicados em terapia gênica
• Beneficiário: Bruna Gregatti de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 19/19719-1 - Matrizes peptídicas bioativas: da estrutura molecular às aplicações biomédicas
• Beneficiário: Lucas Rodrigues de Mello
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/06635-1 - Desenvolvimento de sistemas efetivos baseados de biomateriais para entrega segura e eficiente de macrófagos derivados de iPSC no músculo esquelético para tratar a isquemia de membro
• Beneficiário: Sang Won Han
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/20206-8 - Modulação de monócitos, macrófagos e pericitos pelos genes dos fatores estimuladores de colônia para tratamento de isquemia de membros em modelo murino
• Beneficiário: Sang Won Han
• Modalidade de apoio: Auxílio à Pesquisa - Temático