Multifaceted effect of caffeic acid against Strept... - BV FAPESP
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Multifaceted effect of caffeic acid against Streptococcus mutans infection: microbicidal and immunomodulatory agent in macrophages

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Autor(es):
Sorgi, Carlos Arterio [1] ; de Campos Chaves Lamarque, Giuliana [2] ; Verri, Maraisa P. [3] ; Nelson-Filho, Paulo [2] ; Faccioli, Lucia Helena [3] ; Paula-Silva, Francisco Wanderley Garcia [3, 2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Chem, Sch Philosophy Sci & Letters Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Pediat Clin, Sch Dent Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Archives of Microbiology; v. 203, n. 6, p. 2979-2987, AUG 2021.
Citações Web of Science: 0
Resumo

The aim of the present study was to investigate the effects of caffeic acid in the interface between the antimicrobial and anti-inflammatory function in macrophage response against S. mutans. S. mutans (10(8) cfu/mL) were incubated with caffeic acid to determinate the half-maximal inhibitory concentration (IC50) and macrophage cells were incubated with caffeic acid to determinate cell viability and toxicity. Anti-inflammatory effects were measured by nitrite accumulation, TNF-alpha and PGE(2) production, and NF-kB phosphorylation, and S. mutans survival following internalization by macrophages was investigated. We found that caffeic acid presented antimicrobial activity against S. mutans (IC50 = 2.938 +/- 0.1225 mM) without exerting cytotoxicity. Caffeic acid inhibited nitrite, TNF-alpha and PGE(2) production by the NF-kB dependent pathway, indicating an immunomodulatory property. Caffeic acid also contributed to macrophage bacteria clearance activity. In summary, caffeic acid presented antimicrobial activity against S. mutans and anti-inflammatory effects in macrophages. (AU)

Processo FAPESP: 19/00204-1 - Papel do fator de necrose tumoral-alfa na inflamação e no reparo pulpar e periapical
Beneficiário:Francisco Wanderley Garcia de Paula e Silva
Modalidade de apoio: Auxílio à Pesquisa - Regular