Multifaceted effect of caffeic acid against Streptococcus mutans infection: microbicidal and immunomodulatory agent in macrophages

Author(s)

Sorgi, Carlos Arterio ^[1] ; de Campos Chaves Lamarque, Giuliana ^[2] ; Verri, Maraisa P. ^[3] ; Nelson-Filho, Paulo ^[2] ; Faccioli, Lucia Helena ^[3] ; Paula-Silva, Francisco Wanderley Garcia ^{[3, 2]}

Affiliation

^[1] Univ Sao Paulo, Dept Chem, Sch Philosophy Sci & Letters Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Pediat Clin, Sch Dent Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

• Document type: Journal article
• Source: Archives of Microbiology; v. 203, n. 6, p. 2979-2987, AUG 2021.
• Web of Science Citations: 0

Abstract

The aim of the present study was to investigate the effects of caffeic acid in the interface between the antimicrobial and anti-inflammatory function in macrophage response against S. mutans. S. mutans (10(8) cfu/mL) were incubated with caffeic acid to determinate the half-maximal inhibitory concentration (IC50) and macrophage cells were incubated with caffeic acid to determinate cell viability and toxicity. Anti-inflammatory effects were measured by nitrite accumulation, TNF-alpha and PGE(2) production, and NF-kB phosphorylation, and S. mutans survival following internalization by macrophages was investigated. We found that caffeic acid presented antimicrobial activity against S. mutans (IC50 = 2.938 +/- 0.1225 mM) without exerting cytotoxicity. Caffeic acid inhibited nitrite, TNF-alpha and PGE(2) production by the NF-kB dependent pathway, indicating an immunomodulatory property. Caffeic acid also contributed to macrophage bacteria clearance activity. In summary, caffeic acid presented antimicrobial activity against S. mutans and anti-inflammatory effects in macrophages. (AU)

• FAPESP's process: 19/00204-1 - Role of tumor necrosis factor-alfa in pulpal and periapical inflammation and repair
• Grantee: Francisco Wanderley Garcia de Paula e Silva
• Support Opportunities: Regular Research Grants