Serum activity of matrix metalloproteinase-2 and-9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study

Autor(es)

Fernanda Garcia-Salazar, Luisa ^{[1, 2]} ; Matos Ribeiro, Jean Alex ^[1] ; Emanuel Cunha, Jonathan ^[1] ; Marcia Mattiello, Stela ^[1] ; Luiz Russo, Thiago ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Dept Physiotherapy, Rodovia Washington Luis Km 235, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Rosario, Sch Med & Hlth Sci, Rehabil Sci Res Grp, Physiotherapy Program, Bogota - Colombia

• Tipo de documento: Artigo Científico
• Fonte: Topics in Stroke Rehabilitation; AUG 2021.
• Citações Web of Science: 0

Resumo

Background High concentrations of matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) are associated with inflammation early after stroke. In chronic stages, the elevation of some cytokines is related to the presence of co-morbid conditions in these individuals. In addition to this, some characteristics such as sensorimotor impairment, atrophy, and sedentary lifestyle predispose the system to an inflammatory response. Objective To quantify MMP-2 and -9 serum activity in chronic post-stroke individuals and correlate it with variables of physical activity level, body composition, functional and walking capacity, and with inflammatory biomarkers. Additionally, gelatinase activity was characterized according to motor impairment. Methods Fourteen patients with stroke onset >6 months and seven healthy individuals were enrolled in this study. The clinical assessment included: body composition, measure by bioelectrical impedance analyzer; Fugl-Meyer Motor Assessment Scale; six-minute and ten-meter walk tests, and physical activity level assessed by the StepWatch (R) Activity Monitor. Blood samples were collected from antecubital vein and serum MMP-2 and -9 activity was analyzed using gelatin Zymography, and the TNF alpha, IL-6, IL-1 beta, IL-10 biomarkers using ELISA kits. Results Chronic post-stroke individuals presented an increased activity of MMP-2 and -9 compared to healthy individuals. Positive correlations with time and steps in low cadence and negative ones with medium cadence and peak activity index were observed. According to the motor impairment, the MMP-2 activity was increased in the mild-moderate group compared to the control group. Conclusion Increased gelatinases in chronic post-stroke individuals could describe an inflammation process related to the limited capacity of walking in high intensities. (AU)

• Processo FAPESP: 17/13655-6 - Sedentarismo e disfunção arterial em indivíduos pós-acidente vascular cerebral crônico
• Beneficiário: Thiago Luiz de Russo
• Modalidade de apoio: Auxílio à Pesquisa - Regular