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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Serum activity of matrix metalloproteinase-2 and-9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study

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Author(s):
Fernanda Garcia-Salazar, Luisa [1, 2] ; Matos Ribeiro, Jean Alex [1] ; Emanuel Cunha, Jonathan [1] ; Marcia Mattiello, Stela [1] ; Luiz Russo, Thiago [1]
Total Authors: 5
Affiliation:
[1] Fed Univ Sao Carlos UFSCar, Dept Physiotherapy, Rodovia Washington Luis Km 235, CP 676, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Rosario, Sch Med & Hlth Sci, Rehabil Sci Res Grp, Physiotherapy Program, Bogota - Colombia
Total Affiliations: 2
Document type: Journal article
Source: Topics in Stroke Rehabilitation; AUG 2021.
Web of Science Citations: 0
Abstract

Background High concentrations of matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) are associated with inflammation early after stroke. In chronic stages, the elevation of some cytokines is related to the presence of co-morbid conditions in these individuals. In addition to this, some characteristics such as sensorimotor impairment, atrophy, and sedentary lifestyle predispose the system to an inflammatory response. Objective To quantify MMP-2 and -9 serum activity in chronic post-stroke individuals and correlate it with variables of physical activity level, body composition, functional and walking capacity, and with inflammatory biomarkers. Additionally, gelatinase activity was characterized according to motor impairment. Methods Fourteen patients with stroke onset >6 months and seven healthy individuals were enrolled in this study. The clinical assessment included: body composition, measure by bioelectrical impedance analyzer; Fugl-Meyer Motor Assessment Scale; six-minute and ten-meter walk tests, and physical activity level assessed by the StepWatch (R) Activity Monitor. Blood samples were collected from antecubital vein and serum MMP-2 and -9 activity was analyzed using gelatin Zymography, and the TNF alpha, IL-6, IL-1 beta, IL-10 biomarkers using ELISA kits. Results Chronic post-stroke individuals presented an increased activity of MMP-2 and -9 compared to healthy individuals. Positive correlations with time and steps in low cadence and negative ones with medium cadence and peak activity index were observed. According to the motor impairment, the MMP-2 activity was increased in the mild-moderate group compared to the control group. Conclusion Increased gelatinases in chronic post-stroke individuals could describe an inflammation process related to the limited capacity of walking in high intensities. (AU)

FAPESP's process: 17/13655-6 - Sedentary behaviour and arterial dysfunction in chronic post-stroke subjects
Grantee:Thiago Luiz de Russo
Support Opportunities: Regular Research Grants