Raman and Fluorescence Profiles Modifications of Muscular and Adipose Tissues Exposed to Low Energy X-ray Beams

Autor(es)

Santos, Noemy R. ^[1] ; Kunzel, Roseli ^[1] ; Freitas, Marcelo B. ^[2] ; Levenhagen, Ronaldo S. ^[1] ; Marques, Ana Paula de A. ^[3] ; Courrol, Lilia C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Fis, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Dept Biofis, Escola Paulista Med, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Dept Quim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Spectroscopy; v. 75, n. 9 FEB 2021.
• Citações Web of Science: 0

Resumo

This work aims to investigate changes induced by low-energy radiation in adipose and muscular tissues employing autofluorescence and Raman spectroscopic techniques. X-ray beams expositions with 25 and 35 kV at 0.11, 1.1, and 2.1 Gy radiation dose levels were applied. Changes in Raman line intensities at specific bands assigned to collagen, proteins, and lipids were observed. Autofluorescent analysis exhibit variations in the collagen and nicotinamide adenine dinucleotide emission, resulting from the structural modifications, variations on the reduced/oxidized fluorophores equilibrium followed by radiation exposure. Results show that Raman and fluorescence spectroscopy are suitable techniques to evaluate radiation effects on biomolecules even at low radiation doses and energies. (AU)

• Processo FAPESP: 17/23686-6 - Utilização da Espectroscopia de Fluorescência na Detecção de Contaminação em Alimentos
• Beneficiário: Lilia Coronato Courrol
• Modalidade de apoio: Auxílio à Pesquisa - Regular