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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Raman and Fluorescence Profiles Modifications of Muscular and Adipose Tissues Exposed to Low Energy X-ray Beams

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Author(s):
Santos, Noemy R. [1] ; Kunzel, Roseli [1] ; Freitas, Marcelo B. [2] ; Levenhagen, Ronaldo S. [1] ; Marques, Ana Paula de A. [3] ; Courrol, Lilia C. [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Fis, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Biofis, Escola Paulista Med, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Quim, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Applied Spectroscopy; v. 75, n. 9 FEB 2021.
Web of Science Citations: 0
Abstract

This work aims to investigate changes induced by low-energy radiation in adipose and muscular tissues employing autofluorescence and Raman spectroscopic techniques. X-ray beams expositions with 25 and 35 kV at 0.11, 1.1, and 2.1 Gy radiation dose levels were applied. Changes in Raman line intensities at specific bands assigned to collagen, proteins, and lipids were observed. Autofluorescent analysis exhibit variations in the collagen and nicotinamide adenine dinucleotide emission, resulting from the structural modifications, variations on the reduced/oxidized fluorophores equilibrium followed by radiation exposure. Results show that Raman and fluorescence spectroscopy are suitable techniques to evaluate radiation effects on biomolecules even at low radiation doses and energies. (AU)

FAPESP's process: 17/23686-6 - Use of fluorescence spectroscopy on detection and control of contamination in food
Grantee:Lilia Coronato Courrol
Support Opportunities: Regular Research Grants