mergence of GES-19-producing Pseudomonas aeruginosa exoU plus belonging to the global high-risk clone ST235 in cystic fibrosis infectio

Autor(es)

Rosales-Reyes, Roberto ^[1] ; Esposito, Fernanda ^[2] ; Fontana, Herrison ^[2] ; Lezana-Fernandez, Jose Luis ^[3] ; Lascurain, Ricardo ^[4] ; De la Cruz, Miguel A. ^[5] ; Fuga, Bruna ^{[6, 2]} ; Lincopan, Nilton ^{[6, 2]} ; Santos-Preciado, Jose Ignacio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Mexico City, DF - Mexico

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Analises Clin, Sao Paulo - Brazil

^[3] Hosp Infantil Mexico Dr Federico Gomez, Clin Fibrosis Quist, Lab Fisiologia Pulm, Mexico City, DF - Mexico

^[4] Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City, DF - Mexico

^[5] Inst Mexicano Segura Social, Hosp Pediat, Ctr Med Nacl Siglo 21, Unidad Invest Medial Enfermedades Infecciosas & P, Mexico City, DF - Mexico

^[6] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 101, n. 1 SEP 2021.
• Citações Web of Science: 0

Resumo

The emergence of high-risk clones of priority pathogens exhibiting convergence of antimicrobial resistance and virulence is a critical issue worldwide. In a previous study, an extensively drug-resistant Pseudomonas aeruginosa was isolated from a chronically colonized pediatric patient with cystic fibrosis (CF). In this study, we analyzed genomic data of this strain (CF023-Psa42), extracting clinically and epidemiologically relevant information (i.e., the antimicrobial resistome, virulome, and sequence type). In this regard, we report the emergence of GES-19 (extended-spectrum beta-lactamase)-producing P. aeruginosa with genotype exoU+. The CF023-Psa42 strain exhibited a broad resistome, belonging to the international high-risk clone sequence type ST235. The bla(GES-19) gene was located on a class 1 integron, along to aac(6')-33, aac(60)-Ib-cr, bla(OXA-2), aadA1, sul1, and qacE Delta 1 resistance genes. Relevant virulence genes such as lasA (proteolysis and elastolysis), toxA (exotoxin A), alg (alginate biosynthesis operon), and exoU (toxin of type III secretion systems) were predicted. Our findings reveal the convergence of broad resistome and virulome in P. aeruginosa ST235. Genomic surveillance is essential to monitor the emergence and dissemination of priority pathogens with epidemiological success. (C) 2021 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 19/15578-4 - Viruloma e patogenicidade de linhagens bacterianas prioritárias em saúde única resistentes a carbapenêmicos e polimixinas
• Beneficiário: Fernanda Ribeiro dos Santos Esposito
• Modalidade de apoio: Bolsas no Brasil - Doutorado