ellulolytic enzymes production guided by morphology engineerin

Autor(es)

Buffo, Mariane M. ^[1] ; Ferreira, Andre L. Z. ^[1] ; Almeida, Renata M. R. G. ^[2] ; Farinas, Cristiane S. ^{[1, 3]} ; Badino, Alberto C. ^[1] ; Ximenes, Eduardo A. ^[4] ; Ladisch, Michael R. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Alagoas, BR-57072970 Maceio, AL - Brazil

^[3] Embrapa Instrumentat, Rua 15 Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil

^[4] Purdue Univ, W Lafayette, IN 47907 - USA

• Tipo de documento: Artigo Científico
• Fonte: Enzyme and Microbial Technology; v. 149, SEP 2021.
• Citações Web of Science: 0

Resumo

Endoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. niger morphology changes, endoglucanase production is enhanced under conditions of lower stress and by morphology that results in pellets. beta-glucosidase production is enhanced under dispersed morphology, which results in up to fourfold increase of this enzyme production under the tested experimental conditions. A morphologic scale (Y) is proposed based on a form factor that considers the size and frequency of each morphology class, and that points to conditions that result in high selectivity for either endoglucanase or beta-glucosidase production. An equation proposed to relate enzyme activity to morphology provides a useful tool for tuning enzyme production of A. niger, where morphology is a first indication of relative enzyme activities in a fermentation broth. (AU)

• Processo FAPESP: 18/11405-5 - Fermentação Alcoólica Extrativa em Biorreatores Pneumáticos com Arraste de Etanol por CO2 Integrada a Etapas de Recuperação
• Beneficiário: Alberto Colli Badino Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular