Sensitive LC-MS/MS method for quantification of rivaroxaban in plasma: Application to pharmacokinetic studies

Autor(es)

de Oliveira, Alexandre Cavenatti ^[1] ; Davanco, Marcelo Gomes ^[1] ; de Campos, Daniel Rossi ^[1] ; Godoy Sanches, Pedro Henrique ^[2] ; Goncalves Cirino, Joao Pedro ^[2] ; Carvalho, Patricia de Oliveira ^{[2, 1]} ; Antonio, Marcia Aparecida ^{[1, 3]} ; Coelho, Edvaldo Capobiango ^[3] ; Porcari, Andreia M. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Francisco, Hlth Sci Postgrad Program, Ave Sao Francisco de Assis 218, BR-12916900 Braganca Paulista, SP - Brazil

^[2] Univ Sao Francisco, Lab Multidisciplinary Res, Braganca Paulista, SP - Brazil

^[3] Univ Sao Francisco, Integrated Unit Pharmacol & Gastroenterol UNIFAG, Braganca Paulista, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICAL CHROMATOGRAPHY; v. 35, n. 9 SEP 2021.
• Citações Web of Science: 0

Resumo

Rivaroxaban is an anticoagulant (orally active direct Xa inhibitor) considered to reduce the risk of stroke and systemic embolism and treat deep vein thrombosis, pulmonary embolism, and other cardiovascular complications. Bioanalytical methods for rivaroxaban quantification in plasma are necessary for application in pharmacokinetic studies, as well as in drug therapeutic monitoring. In this work, we developed and validated a sensitive bioanalytical method using LC-MS/MS for rivaroxaban quantification in human plasma using an one-step liquid-liquid extraction. The linear concentration range was 1-600 ng/mL. The bioanalytical method was also applied to pharmacokinetic studies in healthy volunteers under fasting and fed conditions. The results demonstrated that the method is rapid, sensitive, and adequate for application in pharmacokinetic studies. (AU)

• Processo FAPESP: 19/04314-6 - Imageamento e metabolômica por espectrometria de massas aplicada ao estudo de resistência à terapia neoadjuvante para tratamento de câncer de mama: busca por um teste preditivo
• Beneficiário: Andréia de Melo Porcari
• Modalidade de apoio: Auxílio à Pesquisa - Regular